Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 734541..735158 Replicon chromosome
Accession NZ_CP059961
Organism Pectobacterium brasiliense strain IPO:4057 NAK:249

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A39_RS03295 Protein ID WP_010281507.1
Coordinates 734541..734720 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A39_RS03300 Protein ID WP_014916995.1
Coordinates 734748..735158 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A39_RS03270 729782..730942 - 1161 WP_039465321.1 cystathionine gamma-synthase -
H5A39_RS03275 731130..731447 + 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A39_RS03280 731681..732478 + 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A39_RS03285 732488..733450 + 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A39_RS03290 733447..734457 + 1011 WP_119870817.1 iron ABC transporter permease -
H5A39_RS03295 734541..734720 + 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A39_RS03300 734748..735158 + 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A39_RS03305 735255..735470 - 216 WP_010281510.1 50S ribosomal protein L31 -
H5A39_RS03310 735744..737942 + 2199 WP_104209601.1 primosomal protein N' -
H5A39_RS03315 738251..739294 + 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169586 WP_010281507.1 NZ_CP059961:734541-734720 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169586 NZ_CP076605:c67475-67167 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGATCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCGG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169586 WP_014916995.1 NZ_CP059961:734748-735158 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169586 NZ_CP076605:c67714-67478 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References