Detailed information of TA system TA169579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1938622..1939148
• Replicon: chromosome
• Accession: NZ_CP059960
• Organism: Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A37_RS08755
• Protein ID: WP_119871147.1
• Coordinates: 1938622..1938927 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: H5A37_RS08760
• Protein ID: WP_015855076.1
• Coordinates: 1938930..1939148 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A37_RS08725	1933623..1933877	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A37_RS08730	1933981..1934223	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A37_RS08735	1934563..1934856	+	294	WP_038921611.1	DUF1778 domain-containing protein	-
H5A37_RS08740	1934853..1935347	+	495	WP_119871144.1	GNAT family N-acetyltransferase	-
H5A37_RS08745	1935773..1937494	+	1722	WP_119871145.1	AAA family ATPase	-
H5A37_RS08750	1937580..1938422	+	843	WP_119871146.1	DNA adenine methylase	-
H5A37_RS08755	1938622..1938927	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
H5A37_RS08760	1938930..1939148	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H5A37_RS08765	1939207..1939950	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A37_RS08770	1940043..1940393	+	351	WP_158586005.1	hypothetical protein	-
H5A37_RS08775	1940847..1941188	-	342	WP_119871225.1	hypothetical protein	-
H5A37_RS08780	1941374..1941568	+	195	WP_044209559.1	DUF3950 domain-containing protein	-
H5A37_RS08785	1941684..1942946	-	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1934307..1943123	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T169579 WP_119871147.1 NZ_CP059960:c1938927-1938622 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T169579 NZ_CP076596:c2927331-2927113 [Erwinia amylovora]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT169579 WP_015855076.1 NZ_CP059960:c1939148-1938930 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT169579 NZ_CP076596:c2927740-2927351 [Erwinia amylovora]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46