Detailed information of TA system TA169572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1258445..1260033
• Replicon: chromosome
• Accession: NZ_CP059960
• Organism: Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H5A37_RS05655
• Protein ID: WP_119870763.1
• Coordinates: 1258445..1259764 (-)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H5A37_RS05660
• Protein ID: WP_103943261.1
• Coordinates: 1259764..1260033 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A37_RS05630	1254542..1254682	+	141	WP_005976668.1	50S ribosomal protein L34	-
H5A37_RS05635	1254701..1255060	+	360	WP_010281235.1	ribonuclease P protein component	-
H5A37_RS05640	1255024..1255281	+	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
H5A37_RS05645	1255284..1256918	+	1635	WP_010281240.1	membrane protein insertase YidC	-
H5A37_RS05650	1257048..1258412	+	1365	WP_119870764.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
H5A37_RS05655	1258445..1259764	-	1320	WP_119870763.1	type II toxin-antitoxin system HipA family toxin	Toxin
H5A37_RS05660	1259764..1260033	-	270	WP_103943261.1	helix-turn-helix domain-containing protein	Antitoxin
H5A37_RS05665	1260226..1260492	-	267	Protein_1090	alpha/beta hydrolase	-
H5A37_RS05670	1260621..1261472	-	852	WP_119870762.1	SDR family oxidoreductase	-
H5A37_RS05675	1261615..1262025	+	411	WP_094431545.1	helix-turn-helix transcriptional regulator	-
H5A37_RS05680	1262056..1262607	-	552	WP_119870761.1	TetR/AcrR family transcriptional regulator	-
H5A37_RS05685	1262711..1264213	+	1503	WP_119870760.1	MFS transporter	-
H5A37_RS05690	1264336..1264995	-	660	WP_014917137.1	DUF799 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49397.67 Da
• Isoelectric Point: 6.6897

>T169572 WP_119870763.1 NZ_CP059960:c1259764-1258445 [Pectobacterium brasiliense]
MAALDVYMNGYRVGILTKTGSGAHHFSYDTNWLGLLGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIEYKTLSEQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNVTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVYPERDRYRFMQTQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAQTV
DTVMTTVGSQLPVDFPTHISEAILAGLQARSRRLMSGWE

Nucleotide

• Length: 1320 bp

>T169572 NZ_CP076595:c1620577-1620302 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10062.40 Da
• Isoelectric Point: 9.7281

>AT169572 WP_103943261.1 NZ_CP059960:c1260033-1259764 [Pectobacterium brasiliense]
MMKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEAS
VASSWKEEW

Nucleotide

• Length: 270 bp

>AT169572 NZ_CP076595:c1620829-1620581 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA