Detailed information of TA system TA169569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1093415..1094049
• Replicon: chromosome
• Accession: NZ_CP059960
• Organism: Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5A37_RS04955
• Protein ID: WP_014917023.1
• Coordinates: 1093774..1094049 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: H5A37_RS04950
• Protein ID: WP_014917022.1
• Coordinates: 1093415..1093777 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A37_RS04930	1088544..1089428	-	885	WP_014917008.1	N-carbamoylputrescine amidase	-
H5A37_RS04935	1089709..1090671	-	963	WP_010281567.1	6-phosphofructokinase	-
H5A37_RS04940	1090879..1091781	-	903	WP_119870811.1	CDF family cation-efflux transporter FieF	-
H5A37_RS04945	1091854..1093041	-	1188	WP_080162913.1	sugar transporter	-
H5A37_RS04950	1093415..1093777	-	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5A37_RS04955	1093774..1094049	-	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5A37_RS04960	1094286..1094810	-	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
H5A37_RS04965	1094964..1095662	+	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
H5A37_RS04970	1095659..1097029	+	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T169569 WP_014917023.1 NZ_CP059960:c1094049-1093774 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T169569 NZ_CP076595:c253362-252991 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT169569 WP_014917022.1 NZ_CP059960:c1093777-1093415 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT169569 NZ_CP076595:c253580-253359 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7