Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1069618..1070235 Replicon chromosome
Accession NZ_CP059960
Organism Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A37_RS04825 Protein ID WP_010281507.1
Coordinates 1069618..1069797 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A37_RS04830 Protein ID WP_014916995.1
Coordinates 1069825..1070235 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A37_RS04800 1064859..1066019 - 1161 WP_039465321.1 cystathionine gamma-synthase -
H5A37_RS04805 1066207..1066524 + 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A37_RS04810 1066758..1067555 + 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A37_RS04815 1067565..1068527 + 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A37_RS04820 1068524..1069534 + 1011 WP_119870817.1 iron ABC transporter permease -
H5A37_RS04825 1069618..1069797 + 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A37_RS04830 1069825..1070235 + 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A37_RS04835 1070332..1070547 - 216 WP_010281510.1 50S ribosomal protein L31 -
H5A37_RS04840 1070821..1073019 + 2199 WP_104209601.1 primosomal protein N' -
H5A37_RS04845 1073328..1074371 + 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169568 WP_010281507.1 NZ_CP059960:1069618-1069797 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169568 NZ_CP076595:c193747-193568 [Erwinia amylovora]
ATGGATAGCAGGAATGCAATAGCAATGATAGAGGCCGATGGATGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATCCCCAAAAGGACATACCGCTACCAACGCTGAAAAGCATTA
AAAAACAGGCGGGGCTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169568 WP_014916995.1 NZ_CP059960:1069825-1070235 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169568 NZ_CP076595:c193545-193135 [Erwinia amylovora]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCCGATATTCCAGGCTGCTTCTCTGG
CGGCGACAATTATCAGGATGCGATTGAGAGCGCGCGTGAGGCTATCGACGCGCATATCGAATTACTGGTGGAAGATGGCG
AAGCGGTGCCGGAAGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGTGTGGTGTGGGCGCTGGTAGAT
GTGGACGTTACCCGGCTGATGGGGAAAGCGGAAAAAATCAATGTTACGCTCCCTTCTTTACTGATCCGCCGTATCGATCA
GTTTGTCGCTGCACATCCTGAGTACGGTAGCCGTTCCGGCTTCCTGTCCCGCGTGGCGGCTGATAAAGTGATTGGTCGCG
AAAAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References