Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 422247..422887 Replicon chromosome
Accession NZ_CP059960
Organism Pectobacterium brasiliense strain IPO:4060 NAK:251

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag H5A37_RS01960 Protein ID WP_039278477.1
Coordinates 422247..422663 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag H5A37_RS01965 Protein ID WP_039278475.1
Coordinates 422660..422887 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A37_RS01940 417832..418818 - 987 WP_040033638.1 quinone oxidoreductase -
H5A37_RS01945 418947..420353 + 1407 WP_010279223.1 replicative DNA helicase -
H5A37_RS01950 420395..421477 + 1083 WP_119872014.1 alanine racemase -
H5A37_RS01955 421470..422108 + 639 WP_039517591.1 hypothetical protein -
H5A37_RS01960 422247..422663 - 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
H5A37_RS01965 422660..422887 - 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5A37_RS01970 423804..424283 + 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
H5A37_RS01975 424534..425148 + 615 WP_119872013.1 YitT family protein -
H5A37_RS01980 425212..426405 + 1194 WP_119872012.1 aspartate/tyrosine/aromatic aminotransferase -
H5A37_RS01985 426489..426986 + 498 WP_039278469.1 M48 family metallopeptidase -
H5A37_RS01990 427111..427590 + 480 WP_014916572.1 Hcp family type VI secretion system effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T169567 WP_039278477.1 NZ_CP059960:c422663-422247 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T169567 NZ_CP076594:c3119607-3119287 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT169567 WP_039278475.1 NZ_CP059960:c422887-422660 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT169567 NZ_CP076594:c3119967-3119644 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References