Detailed information of TA system TA169565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2395009..2396045
• Replicon: chromosome
• Accession: NZ_CP059959
• Organism: Pectobacterium brasiliense strain IPO:4062 NAK:237

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H5A40_RS10860
• Protein ID: WP_039283954.1
• Coordinates: 2395470..2396045 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H5A40_RS10855
• Protein ID: WP_039283951.1
• Coordinates: 2395009..2395470 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A40_RS10835	2390038..2390571	+	534	WP_010282870.1	RNA pyrophosphohydrolase	-
H5A40_RS10840	2390584..2392830	+	2247	WP_039283946.1	phosphoenolpyruvate--protein phosphotransferase	-
H5A40_RS10845	2393057..2393923	+	867	WP_010286783.1	prolipoprotein diacylglyceryl transferase	-
H5A40_RS10850	2393960..2394754	+	795	WP_039312476.1	thymidylate synthase	-
H5A40_RS10855	2395009..2395470	+	462	WP_039283951.1	helix-turn-helix domain-containing protein	Antitoxin
H5A40_RS10860	2395470..2396045	+	576	WP_039283954.1	PIN domain-containing protein	Toxin
H5A40_RS10865	2396225..2396728	+	504	WP_039497210.1	prepilin peptidase-dependent protein	-
H5A40_RS10870	2396722..2397348	+	627	WP_052238168.1	prepilin peptidase-dependent protein	-
H5A40_RS10875	2397384..2397800	+	417	WP_205592429.1	DUF2509 family protein	-
H5A40_RS10880	2397787..2398182	+	396	WP_014914413.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21463.88 Da
• Isoelectric Point: 4.7230

>T169565 WP_039283954.1 NZ_CP059959:2395470-2396045 [Pectobacterium brasiliense]
MNHSPYPVVLDACVIYPSLLRDLLMHLGLTGLYQPKWTALIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGF
EQLIPALVLPDADDRHVLAAAIRANAEVIVTANLKDFPATALSEFAVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRK
PPFDVDEYLDCLLRQGLPMTVKELDAYRALI

Nucleotide

• Length: 576 bp

>T169565 NZ_CP076594:c2864083-2863664 [Erwinia amylovora]
GTGGTCCTGTGGCAATGTGAACTGCGGCCATCAAGGCTGGCACAGCGGTTTTCGCTGCTGCTGCATGGGGCAGTGATGCT
GGCGCTGCTATTGCCGACATGGCCCGCCAGCTCTGGCCTGGTGCGGATGCTGTTGCTGGTTTTGGTACTGTTGGAGTGCA
TACGCAGTCGGCGGCGCATTGGTCGTCGCCAGGGGGATATCGCTCTGTTGGAAGGGCATGAGCTACGCTGGCGTCAACGC
GAGTGGTGCATTATTTCCCGTCCGTGGCTGACCGGGCAGGCAATCCTGCTGTTACTACAGGATACTCATGGACAGCGCGA
GCGACTATGGCTGTTCGCCGACGGCATGGAAAAACGTAACTGGCGTCAGCTGCGCCTGCAATTGCTGAACAGTAAAGTAC
AGGGGAATGGCTGGTGCTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17258.82 Da
• Isoelectric Point: 6.2253

>AT169565 WP_039283951.1 NZ_CP059959:2395009-2395470 [Pectobacterium brasiliense]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISILDAHDIAHRIELPTSALTLLMSILGELATGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQELGFY

Nucleotide

• Length: 462 bp

>AT169565 NZ_CP076594:c2864330-2864064 [Erwinia amylovora]
ATGGATATTACAAATAAATCACGTATTCACTGGGCATGCCGTCGCGGCATGCGCGAGCTGGATATCTCGATCATGCCCTT
TTTCCAGTATGAATTTGACTCGCTGAACGACAGCGATAAACGGCTGTTCGTTCGCCTGCTGGAGAGCGATGACCCGGACC
TGTTCAACTGGCTGATGAACCACGGCGAGCCGGCAGACGTGGAGCTGAAAAGGATGGTGGCGCTAATCCAACAAAGAAAT
AAACAACGTGGTCCTGTGGCAATGTGA