Detailed information of TA system TA169562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1545132..1545766
• Replicon: chromosome
• Accession: NZ_CP059959
• Organism: Pectobacterium brasiliense strain IPO:4062 NAK:237

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5A40_RS06985
• Protein ID: WP_080162911.1
• Coordinates: 1545132..1545407 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: H5A40_RS06990
• Protein ID: WP_014917022.1
• Coordinates: 1545404..1545766 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A40_RS06960	1540578..1541276	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
H5A40_RS06965	1541430..1541954	+	525	WP_104213552.1	cell-envelope stress modulator CpxP	-
H5A40_RS06970	1542571..1542753	+	183	Protein_1346	hypothetical protein	-
H5A40_RS06975	1542911..1544569	+	1659	WP_039502364.1	hypothetical protein	-
H5A40_RS06980	1544556..1544762	+	207	WP_039502363.1	hypothetical protein	-
H5A40_RS06985	1545132..1545407	+	276	WP_080162911.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5A40_RS06990	1545404..1545766	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5A40_RS06995	1546140..1547327	+	1188	WP_205592137.1	sugar transporter	-
H5A40_RS07000	1547410..1548312	+	903	WP_205592415.1	CDF family cation-efflux transporter FieF	-
H5A40_RS07005	1548520..1549482	+	963	WP_010281567.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10024.61 Da
• Isoelectric Point: 11.1875

>T169562 WP_080162911.1 NZ_CP059959:1545132-1545407 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T169562 NZ_CP076594:c1691158-1690979 [Erwinia amylovora]
ATGGATAGCAGGAATGCAATAGCAATGATAGAGGCCGATGGATGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATCCCCAAAAGGACATACCGCTACCAACGCTGAAAAGCATTA
AAAAACAGGCGGGGCTTTAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT169562 WP_014917022.1 NZ_CP059959:1545404-1545766 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT169562 NZ_CP076594:c1690956-1690546 [Erwinia amylovora]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCCGATATTCCAGGCTGCTTCTCTGG
CGGCGACAATTATCAGGATGCGATTGAGAGCGCGCGTGAGGCTATCGACGCGCATATCGAATTACTGGTGGAAGATGGCG
AAGCGGTGCCGGAAGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGTGTGGTGTGGGCGCTGGTAGAT
GTGGACGTTACCCGGCTGATGGGGAAAGCGGAAAAAATCAATGTTACGCTCCCTTCTTTACTGATCCGCCGTATCGATCA
GTTTGTCGCTGCACATCCTGAGTACGGTAGCCGTTCCGGCTTCCTGTCCCGCGTGGCGGCTGATAAAGTGATTGGTCGCG
AAAAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7