Detailed information of TA system TA169561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1528012..1528763
• Replicon: chromosome
• Accession: NZ_CP059959
• Organism: Pectobacterium brasiliense strain IPO:4062 NAK:237

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H5A40_RS06900
• Protein ID: WP_205592132.1
• Coordinates: 1528281..1528763 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A7V8QCC3
• Locus tag: H5A40_RS06895
• Protein ID: WP_039521400.1
• Coordinates: 1528012..1528290 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A40_RS06865	1523171..1524085	-	915	WP_014917036.1	NAD(P)-dependent oxidoreductase	-
H5A40_RS06870	1524424..1525182	+	759	WP_039465435.1	DeoR/GlpR family DNA-binding transcription regulator	-
H5A40_RS06875	1525403..1525801	+	399	WP_131591699.1	hypothetical protein	-
H5A40_RS06880	1525798..1526646	-	849	WP_205592131.1	DUF4225 domain-containing protein	-
H5A40_RS06885	1526648..1527125	-	478	Protein_1329	Hcp family type VI secretion system effector	-
H5A40_RS06890	1527486..1527902	+	417	WP_205592414.1	hypothetical protein	-
H5A40_RS06895	1528012..1528290	+	279	WP_039521400.1	DUF1778 domain-containing protein	Antitoxin
H5A40_RS06900	1528281..1528763	+	483	WP_205592132.1	GNAT family N-acetyltransferase	Toxin
H5A40_RS06905	1528896..1530230	+	1335	WP_205592133.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H5A40_RS06910	1530303..1531190	+	888	WP_010281599.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H5A40_RS06915	1531216..1532061	+	846	WP_010281598.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H5A40_RS06920	1532087..1533160	+	1074	WP_205592134.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (39-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17495.28 Da
• Isoelectric Point: 9.0439

>T169561 WP_205592132.1 NZ_CP059959:1528281-1528763 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGVSRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Nucleotide

• Length: 483 bp

>T169561 NZ_CP076594:718586-718822 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10609.08 Da
• Isoelectric Point: 4.4356

>AT169561 WP_039521400.1 NZ_CP059959:1528012-1528290 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITAERQAR
INDLMSRKSPWE

Nucleotide

• Length: 279 bp

>AT169561 NZ_CP076594:718369-718596 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A7V8QCC3