Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3259158..3259798 Replicon chromosome
Accession NZ_CP059958
Organism Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag H5A29_RS14430 Protein ID WP_039278477.1
Coordinates 3259382..3259798 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag H5A29_RS14425 Protein ID WP_039278475.1
Coordinates 3259158..3259385 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A29_RS14400 3254455..3254934 - 480 WP_014916572.1 Hcp family type VI secretion system effector -
H5A29_RS14405 3255059..3255556 - 498 WP_039278469.1 M48 family metallopeptidase -
H5A29_RS14410 3255640..3256833 - 1194 WP_119872012.1 aspartate/tyrosine/aromatic aminotransferase -
H5A29_RS14415 3256897..3257511 - 615 WP_119872013.1 YitT family protein -
H5A29_RS14420 3257762..3258241 - 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
H5A29_RS14425 3259158..3259385 + 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5A29_RS14430 3259382..3259798 + 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
H5A29_RS14435 3259937..3260575 - 639 WP_039517591.1 hypothetical protein -
H5A29_RS14440 3260568..3261650 - 1083 WP_119872014.1 alanine racemase -
H5A29_RS14445 3261692..3263098 - 1407 WP_010279223.1 replicative DNA helicase -
H5A29_RS14450 3263227..3264213 + 987 WP_040033638.1 quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T169557 WP_039278477.1 NZ_CP059958:3259382-3259798 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T169557 NZ_CP076592:2581602-2582021 [Erwinia amylovora]
GTGGTCCTGTGGCAATGTGAACTGCGGCCATCAAGGCTGGCACAGCGGTTTTCGCTGCTGCTGCATGGGGCAGTGATGCT
GGCGCTGCTATTGCCGACATGGCCCGCCAGCTCTGGCCTGGTGCGGATGCTGTTGCTGGTTTTGGTACTGTTGGAGTGCA
TACGCAGTCGGCGGCGCATTGGTCGTCGCCAGGGGGATATCGCTCTGTTGGAAGGGCATGAGCTACGCTGGCGTCAACGC
GAGTGGTGCATTATTTCCCGTCCGTGGCTGACCGGGCAGGCAATCCTGCTGTTACTACAGGATACTCATGGACAGCGCGA
GCGACTATGGCTGTTCGCCGACGGCATGGAAAAACGTAACTGGCGTCAGCTGCGCCTGCAATTGCTGAACAGTAAAGTAC
AGGGGAATGGCTGGTGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT169557 WP_039278475.1 NZ_CP059958:3259158-3259385 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT169557 NZ_CP076592:2581355-2581621 [Erwinia amylovora]
ATGGATATTACAAATAAATCACGTATTCACTGGGCATGCCGTCGCGGCATGCGCGAGCTGGATATCTCGATCATGCCCTT
TTTCCAGTATGAATTTGACTCGCTGAACGACAGCGATAAACGGCTGTTCGTTCGCCTGCTGGAGAGCGATGACCCGGACC
TGTTCAACTGGCTGATGAACCACGGCGAGCCGGCAGACGTGGAGCTGAAAAGGATGGTGGCGCTAATCCAACAAAGAAAT
AAACAACGTGGTCCTGTGGCAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References