Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2611815..2612432 Replicon chromosome
Accession NZ_CP059958
Organism Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A29_RS11560 Protein ID WP_010281507.1
Coordinates 2612253..2612432 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A29_RS11555 Protein ID WP_014916995.1
Coordinates 2611815..2612225 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A29_RS11540 2607679..2608722 - 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -
H5A29_RS11545 2609031..2611229 - 2199 WP_104209601.1 primosomal protein N' -
H5A29_RS11550 2611503..2611718 + 216 WP_010281510.1 50S ribosomal protein L31 -
H5A29_RS11555 2611815..2612225 - 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A29_RS11560 2612253..2612432 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A29_RS11565 2612516..2613526 - 1011 WP_119870817.1 iron ABC transporter permease -
H5A29_RS11570 2613523..2614485 - 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A29_RS11575 2614495..2615292 - 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A29_RS11580 2615526..2615843 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A29_RS11585 2616031..2617191 + 1161 WP_039465321.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169556 WP_010281507.1 NZ_CP059958:c2612432-2612253 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169556 NZ_CP076592:2327693-2327968 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169556 WP_014916995.1 NZ_CP059958:c2612225-2611815 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169556 NZ_CP076592:2327441-2327689 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References