Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2573936..2574687 Replicon chromosome
Accession NZ_CP059958
Organism Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)


Gene name ataT Uniprot ID A0A433N1V3
Locus tag H5A29_RS11360 Protein ID WP_010281603.1
Coordinates 2574205..2574687 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A0M2EWB4
Locus tag H5A29_RS11355 Protein ID WP_039317395.1
Coordinates 2573936..2574214 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A29_RS11325 2569095..2570009 - 915 WP_119870802.1 NAD(P)-dependent oxidoreductase -
H5A29_RS11330 2570347..2571105 + 759 WP_119870824.1 DeoR/GlpR family DNA-binding transcription regulator -
H5A29_RS11335 2571326..2571724 + 399 WP_127110965.1 hypothetical protein -
H5A29_RS11340 2571721..2572569 - 849 WP_119870804.1 DUF4225 domain-containing protein -
H5A29_RS11345 2572571..2573050 - 480 WP_039511462.1 Hcp family type VI secretion system effector -
H5A29_RS11350 2573410..2573826 + 417 WP_119870805.1 hypothetical protein -
H5A29_RS11355 2573936..2574214 + 279 WP_039317395.1 DUF1778 domain-containing protein Antitoxin
H5A29_RS11360 2574205..2574687 + 483 WP_010281603.1 GNAT family N-acetyltransferase Toxin
H5A29_RS11365 2574820..2576154 + 1335 WP_119870806.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
H5A29_RS11370 2576227..2577114 + 888 WP_010281599.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
H5A29_RS11375 2577140..2577985 + 846 WP_010281598.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
H5A29_RS11380 2578011..2579084 + 1074 WP_119870807.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(39-144)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17509.30 Da        Isoelectric Point: 9.0439

>T169554 WP_010281603.1 NZ_CP059958:2574205-2574687 [Pectobacterium brasiliense]
MGITAPEPLSSSHNLAEFCSQEPSLNEWLKKKALKNHSAGISRVYVICAEGTHQVIGYYCLSTGSVQRNTAPGAYRRNAP
ESLPVIVLGRLAIDQAYSGKGLGVALLKDAIFRTENIALQVGVRALVVHALDENVRNFYLKFAFDPSPVQPLMLLYPIKI

Download         Length: 483 bp

>T169554 NZ_CP076592:c923032-922796 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10667.12 Da        Isoelectric Point: 4.3611

>AT169554 WP_039317395.1 NZ_CP059958:2573936-2574214 [Pectobacterium brasiliense]
MKPETKEAPINIRAKASQRDLIDMAANLVSKSRTDFMLEAACREAQDILLDQRLFILDNEQFDEFLAELDAPITEERQAR
INDLMSRKSPWE

Download         Length: 279 bp

>AT169554 NZ_CP076592:c923249-923022 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A433N1V3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2EWB4

References