Detailed information of TA system TA169546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1746713..1747497
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5A29_RS07635
• Protein ID: WP_119871144.1
• Coordinates: 1746713..1747207 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: H5A29_RS07640
• Protein ID: WP_038921611.1
• Coordinates: 1747204..1747497 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS07610	1742110..1742853	+	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A29_RS07615	1742912..1743130	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
H5A29_RS07620	1743133..1743438	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
H5A29_RS07625	1743638..1744480	-	843	WP_119871146.1	DNA adenine methylase	-
H5A29_RS07630	1744566..1746287	-	1722	WP_119871145.1	AAA family ATPase	-
H5A29_RS07635	1746713..1747207	-	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
H5A29_RS07640	1747204..1747497	-	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
H5A29_RS07645	1747837..1748079	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A29_RS07650	1748183..1748437	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A29_RS07655	1748522..1748770	+	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
H5A29_RS07660	1749422..1751176	-	1755	WP_119871142.1	AIPR family protein	-
H5A29_RS07665	1751404..1752189	-	786	WP_185893700.1	phosphonate metabolism protein PhnP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1738937..1747753	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T169546 WP_119871144.1 NZ_CP059958:c1747207-1746713 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T169546 NZ_CP076590:c2645734-2645498 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT169546 WP_038921611.1 NZ_CP059958:c1747497-1747204 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169546 NZ_CP076590:c2645951-2645724 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3