Detailed information of TA system TA169545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1742912..1743438
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A29_RS07620
• Protein ID: WP_119871147.1
• Coordinates: 1743133..1743438 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: H5A29_RS07615
• Protein ID: WP_015855076.1
• Coordinates: 1742912..1743130 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS07590	1739114..1740376	+	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-
H5A29_RS07595	1740492..1740686	-	195	WP_044209559.1	DUF3950 domain-containing protein	-
H5A29_RS07600	1740872..1741213	+	342	WP_119871225.1	hypothetical protein	-
H5A29_RS07605	1741667..1742017	-	351	WP_158586005.1	hypothetical protein	-
H5A29_RS07610	1742110..1742853	+	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A29_RS07615	1742912..1743130	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H5A29_RS07620	1743133..1743438	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
H5A29_RS07625	1743638..1744480	-	843	WP_119871146.1	DNA adenine methylase	-
H5A29_RS07630	1744566..1746287	-	1722	WP_119871145.1	AAA family ATPase	-
H5A29_RS07635	1746713..1747207	-	495	WP_119871144.1	GNAT family N-acetyltransferase	-
H5A29_RS07640	1747204..1747497	-	294	WP_038921611.1	DUF1778 domain-containing protein	-
H5A29_RS07645	1747837..1748079	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A29_RS07650	1748183..1748437	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1738937..1747753	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T169545 WP_119871147.1 NZ_CP059958:1743133-1743438 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T169545 NZ_CP076590:1673158-1673337 [Erwinia amylovora]
ATGGATAGCAGGAATGCAATAGCAATGATAGAGGCCGATGGATGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATCCCCAAAAGGACATACCGCTACCAACGCTGAAAAGCATTA
AAAAACAGGCGGGGCTTTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT169545 WP_015855076.1 NZ_CP059958:1742912-1743130 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT169545 NZ_CP076590:1673360-1673770 [Erwinia amylovora]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCCGATATTCCAGGCTGCTTCTCTGG
CGGCGACAATTATCAGGATGCGATTGAGAGCGCGCGTGAGGCTATCGACGCGCATATCGAATTACTGGTGGAAGATGGCG
AAGCGGTGCCGGAAGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGTGTGGTGTGGGCGCTGGTAGAT
GTGGACGTTACCCGGCTGATGGGGAAAGCGGAAAAAATCAATGTTACGCTCCCTTCTTTACTGATCCGCCGTATCGATCA
GTTTGTCGCTGCACATCCTGAGTACGGTAGCCGTTCCGGCTTCCTGTCCCGCGTGGCGGCTGATAAAGTGATTGGTCGCG
AAAAACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46