Detailed information of TA system TA169544

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1560631..1561291
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H5A29_RS06680
• Protein ID: WP_072061666.1
• Coordinates: 1560631..1561044 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: H5A29_RS06685
• Protein ID: WP_005971623.1
• Coordinates: 1561025..1561291 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS06655	1556429..1557757	+	1329	WP_039282120.1	MHS family MFS transporter	-
H5A29_RS06660	1557962..1558348	+	387	WP_048256533.1	acyl-CoA thioesterase	-
H5A29_RS06665	1558691..1559209	+	519	WP_119870590.1	flavodoxin FldB	-
H5A29_RS06670	1559238..1559561	+	324	WP_119870589.1	hypothetical protein	-
H5A29_RS06675	1559627..1560562	-	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
H5A29_RS06680	1560631..1561044	-	414	WP_072061666.1	protein YgfX	Toxin
H5A29_RS06685	1561025..1561291	-	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
H5A29_RS06690	1561527..1562528	+	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
H5A29_RS06695	1562583..1563230	-	648	WP_010279833.1	hemolysin III family protein	-
H5A29_RS06700	1563661..1564269	+	609	WP_010279828.1	HD domain-containing protein	-
H5A29_RS06705	1564324..1565328	+	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T169544 WP_072061666.1 NZ_CP059958:c1561044-1560631 [Pectobacterium brasiliense]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T169544 NZ_CP076590:1613543-1613914 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT169544 WP_005971623.1 NZ_CP059958:c1561291-1561025 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT169544 NZ_CP076590:1613325-1613546 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6