Detailed information of TA system TA169543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1094239..1095275
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H5A29_RS04565
• Protein ID: WP_119871526.1
• Coordinates: 1094239..1094814 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: H5A29_RS04570
• Protein ID: WP_119871525.1
• Coordinates: 1094814..1095275 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS04545	1092102..1092497	-	396	WP_014914413.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
H5A29_RS04550	1092484..1092900	-	417	WP_119871529.1	YgdB family protein	-
H5A29_RS04555	1092936..1093562	-	627	WP_119871528.1	prepilin peptidase-dependent protein	-
H5A29_RS04560	1093556..1094059	-	504	WP_119871527.1	prepilin peptidase-dependent protein	-
H5A29_RS04565	1094239..1094814	-	576	WP_119871526.1	PIN domain-containing protein	Toxin
H5A29_RS04570	1094814..1095275	-	462	WP_119871525.1	helix-turn-helix domain-containing protein	Antitoxin
H5A29_RS04575	1095498..1096292	-	795	WP_039312476.1	thymidylate synthase	-
H5A29_RS04580	1096329..1097195	-	867	WP_010286783.1	prolipoprotein diacylglyceryl transferase	-
H5A29_RS04585	1097422..1099668	-	2247	WP_039283946.1	phosphoenolpyruvate--protein phosphotransferase	-
H5A29_RS04590	1099681..1100214	-	534	WP_010282870.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21493.91 Da
• Isoelectric Point: 4.7230

>T169543 WP_119871526.1 NZ_CP059958:c1094814-1094239 [Pectobacterium brasiliense]
MNHSPYPVVLDACVIYPSLLRDLLMHLGLTGLYQPKWTVLIQEEWQRNLLLNNADVTMDKLRNIEHLMNKALPDANVTGF
EQLIPALVLPDADDRHVLAAAIRANAEVIVTANLKDFPATSLSEFGVEAMHPDEFIADLFDLNSALVLKAVRTSRTAFRK
PPFDVDEYLDCLLRQGLPMTVKELDAYRALI

Nucleotide

• Length: 576 bp

>T169543 NZ_CP076590:c858699-858481 [Erwinia amylovora]
ATGACTGACAAACTTCTAACGAAAACTGACTACCTGATGCGCCTCAGGCGTTGTCGTTCAATCGATACACTTGAACGCGT
GATTGAAAAGAACAAGTACGAATTATCTGATGATGAACTGGCTGTATTTTATTCAGCCGCCGATCACCGTCTTGCTGAAC
TAACCATGAATAAGCTGTACGACAAAGTACCGGTCGCTGTCTGGAAGTTTGTCCGTTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17277.78 Da
• Isoelectric Point: 6.2253

>AT169543 WP_119871525.1 NZ_CP059958:c1095275-1094814 [Pectobacterium brasiliense]
MKNTPLNSLSLPAPKEMEAAIRGQRQLATYLSTKLETQEISIQDAHDIAHRIELPTSALTLLMSILGELAAGNAVQIVPV
QAELTTQEAANILNVSRPHMVKLLEEGHLPFHKTGRHRRVLFADLMQYKEQRENESMQAMQELTDRSQEFGFY

Nucleotide

• Length: 462 bp

>AT169543 NZ_CP076590:c859108-858719 [Erwinia amylovora]
ATGGACGAATACTCACCGAAAAGGCATGATATTGCGCAGCTGAAATTCTTATGTGAAAACCTGTATGATGAAAGTCTGGC
TACGTTAGGCGACAGCCATCATGGCTGGGTTAACGACCCCACCTCAACGAGCAATCTTCAGCTTAACGACCTGATAGAGC
ACATTGCAGCATTCACAATGAATTACAAAATCAAGCATATCGAAGACAGCGATCTGATAAGTCAAATTGATGAGTATCTG
GACGACACCTTTATGCTTTTCAGCAATTACGGTGTCAACAATCTTGACTTACAACGCTGGCAAAAATCAGCGAAGCGATT
GTTTAACATTTTTGCTAAAGAATGCGTAATGAGCCAGATCCAGTCCAGCCATTCATTTAGCAGCCCATAA