Detailed information of TA system TA169541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 355496..356049
• Replicon: chromosome
• Accession: NZ_CP059958
• Organism: Pectobacterium brasiliense strain IPO:4065 NAK:223

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A29_RS01635
• Protein ID: WP_119870392.1
• Coordinates: 355496..355810 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3S1FM98
• Locus tag: H5A29_RS01640
• Protein ID: WP_029729596.1
• Coordinates: 355813..356049 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A29_RS01615	350556..352418	+	1863	WP_119870396.1	type IV secretory system conjugative DNA transfer family protein	-
H5A29_RS01620	352427..353172	+	746	Protein_319	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A29_RS01625	353369..353911	+	543	WP_119870394.1	DUF3916 domain-containing protein	-
H5A29_RS01630	354074..355072	-	999	WP_119870393.1	hypothetical protein	-
H5A29_RS01635	355496..355810	-	315	WP_119870392.1	CcdB family protein	Toxin
H5A29_RS01640	355813..356049	-	237	WP_029729596.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H5A29_RS01645	356369..357313	-	945	WP_119870391.1	DUF1738 domain-containing protein	-
H5A29_RS01650	358084..360393	+	2310	WP_119870906.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	354074..377550	23476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11569.51 Da
• Isoelectric Point: 7.9859

>T169541 WP_119870392.1 NZ_CP059958:c355810-355496 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIMIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKASIDFLIDGF

Nucleotide

• Length: 315 bp

>T169541 NZ_CP076590:246430-246705 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8927.96 Da
• Isoelectric Point: 6.4612

>AT169541 WP_029729596.1 NZ_CP059958:c356049-355813 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT169541 NZ_CP076590:246178-246426 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S1FM98