Detailed information of TA system TA169540

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4913581..4914241
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H5A30_RS21590
• Protein ID: WP_072061666.1
• Coordinates: 4913581..4913994 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: H5A30_RS21595
• Protein ID: WP_005971623.1
• Coordinates: 4913975..4914241 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS21565	4909379..4910707	+	1329	WP_039282120.1	MHS family MFS transporter	-
H5A30_RS21570	4910912..4911298	+	387	WP_048256533.1	acyl-CoA thioesterase	-
H5A30_RS21575	4911641..4912159	+	519	WP_119870590.1	flavodoxin FldB	-
H5A30_RS21580	4912188..4912511	+	324	WP_119870589.1	hypothetical protein	-
H5A30_RS21585	4912577..4913512	-	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
H5A30_RS21590	4913581..4913994	-	414	WP_072061666.1	protein YgfX	Toxin
H5A30_RS21595	4913975..4914241	-	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
H5A30_RS21600	4914477..4915478	+	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
H5A30_RS21605	4915533..4916180	-	648	WP_010279833.1	hemolysin III family protein	-
H5A30_RS21610	4916611..4917219	+	609	WP_010279828.1	HD domain-containing protein	-
H5A30_RS21615	4917274..4918278	+	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T169540 WP_072061666.1 NZ_CP059957:c4913994-4913581 [Pectobacterium brasiliense]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T169540 NZ_CP076590:244815-245135 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT169540 WP_005971623.1 NZ_CP059957:c4914241-4913975 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT169540 NZ_CP076590:244455-244778 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6