Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4239789..4240414 Replicon chromosome
Accession NZ_CP059957
Organism Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)


Gene name Hha Uniprot ID C6DB72
Locus tag H5A30_RS18650 Protein ID WP_005976087.1
Coordinates 4240211..4240414 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q6D809
Locus tag H5A30_RS18645 Protein ID WP_010282247.1
Coordinates 4239789..4240157 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A30_RS18630 4235422..4238550 + 3129 WP_039511040.1 multidrug efflux RND transporter permease subunit -
H5A30_RS18635 4238781..4239029 + 249 WP_039274617.1 type B 50S ribosomal protein L31 -
H5A30_RS18640 4239047..4239190 + 144 WP_005976091.1 type B 50S ribosomal protein L36 -
H5A30_RS18645 4239789..4240157 + 369 WP_010282247.1 Hha toxicity modulator TomB Antitoxin
H5A30_RS18650 4240211..4240414 + 204 WP_005976087.1 hemolysin expression modulator Hha Toxin
H5A30_RS18660 4241280..4241618 + 339 WP_119872104.1 MGMT family protein -
H5A30_RS18665 4241620..4242201 - 582 WP_119872105.1 YbaY family lipoprotein -
H5A30_RS18670 4242414..4243277 + 864 WP_119872106.1 acyl-CoA thioesterase II -
H5A30_RS18675 4243417..4244703 - 1287 WP_185893748.1 ammonium transporter AmtB -
H5A30_RS18680 4244743..4245081 - 339 WP_002208627.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8145.52 Da        Isoelectric Point: 8.9008

>T169538 WP_005976087.1 NZ_CP059957:4240211-4240414 [Pectobacterium brasiliense]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Download         Length: 204 bp

>T169538 NZ_CP076589:2021339-2021518 [Erwinia amylovora]
ATGGATAGCAGGAATGCAATAGCAATGATAGAGGCCGATGGATGGTATCTGGTGAGAGTAAAAGGCAGTCATCACCAGTT
CAAGCACCCAACGAAAAAAGGGCTGGTAACGGTAAAACATCCCCAAAAGGACATACCGCTACCAACGCTGAAAAGCATTA
AAAAACAGGCGGGGCTTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14046.80 Da        Isoelectric Point: 4.4787

>AT169538 WP_010282247.1 NZ_CP059957:4239789-4240157 [Pectobacterium brasiliense]
MDEYTPKHYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENICTPAKT

Download         Length: 369 bp

>AT169538 NZ_CP076589:2021541-2021951 [Erwinia amylovora]
ATGTTATACCCTGTTGCTATTGATAAAGGCGATTCATCCTTCGGCGTTCGCGTACCCGATATTCCAGGCTGCTTCTCTGG
CGGCGACAATTATCAGGATGCGATTGAGAGCGCGCGTGAGGCTATCGACGCGCATATCGAATTACTGGTGGAAGATGGCG
AAGCGGTGCCGGAAGCGACCAGCGTGGAAAACTGGCTGGCCGATCCCGATTATGTTGGTGTGGTGTGGGCGCTGGTAGAT
GTGGACGTTACCCGGCTGATGGGGAAAGCGGAAAAAATCAATGTTACGCTCCCTTCTTTACTGATCCGCCGTATCGATCA
GTTTGTCGCTGCACATCCTGAGTACGGTAGCCGTTCCGGCTTCCTGTCCCGCGTGGCGGCTGATAAAGTGATTGGTCGCG
AAAAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7Z3F0

Antitoxin

Source ID Structure
AlphaFold DB A0A086EI31

References