Detailed information of TA system TA169537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3708431..3708984
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A30_RS16560
• Protein ID: WP_119870392.1
• Coordinates: 3708431..3708745 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A3S1FM98
• Locus tag: H5A30_RS16565
• Protein ID: WP_029729596.1
• Coordinates: 3708748..3708984 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS16540	3703490..3705352	+	1863	WP_119870396.1	type IV secretory system conjugative DNA transfer family protein	-
H5A30_RS16545	3705361..3706107	+	747	WP_119870395.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A30_RS16550	3706304..3706846	+	543	WP_119870394.1	DUF3916 domain-containing protein	-
H5A30_RS16555	3707009..3708007	-	999	WP_119870393.1	hypothetical protein	-
H5A30_RS16560	3708431..3708745	-	315	WP_119870392.1	CcdB family protein	Toxin
H5A30_RS16565	3708748..3708984	-	237	WP_029729596.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H5A30_RS16570	3709304..3710248	-	945	WP_119870391.1	DUF1738 domain-containing protein	-
H5A30_RS16575	3711013..3713322	+	2310	WP_119870906.1	TerB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3683374..3723567	40193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11569.51 Da
• Isoelectric Point: 7.9859

>T169537 WP_119870392.1 NZ_CP059957:c3708745-3708431 [Pectobacterium brasiliense]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIMIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRSQVKASIDFLIDGF

Nucleotide

• Length: 315 bp

>T169537 NZ_CP076589:1961724-1962095 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8927.96 Da
• Isoelectric Point: 6.4612

>AT169537 WP_029729596.1 NZ_CP059957:c3708984-3708748 [Pectobacterium brasiliense]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT169537 NZ_CP076589:1961506-1961727 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S1FM98