Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1548032..1548672 Replicon chromosome
Accession NZ_CP059957
Organism Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag H5A30_RS06990 Protein ID WP_039278477.1
Coordinates 1548256..1548672 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag H5A30_RS06985 Protein ID WP_039278475.1
Coordinates 1548032..1548259 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A30_RS06960 1543329..1543808 - 480 WP_014916572.1 Hcp family type VI secretion system effector -
H5A30_RS06965 1543933..1544430 - 498 WP_039278469.1 M48 family metallopeptidase -
H5A30_RS06970 1544514..1545707 - 1194 WP_119872012.1 aspartate/tyrosine/aromatic aminotransferase -
H5A30_RS06975 1545771..1546385 - 615 WP_119872013.1 YitT family protein -
H5A30_RS06980 1546636..1547115 - 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
H5A30_RS06985 1548032..1548259 + 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5A30_RS06990 1548256..1548672 + 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
H5A30_RS06995 1548811..1549449 - 639 WP_039517591.1 hypothetical protein -
H5A30_RS07000 1549442..1550524 - 1083 WP_119872014.1 alanine racemase -
H5A30_RS07005 1550566..1551972 - 1407 WP_010279223.1 replicative DNA helicase -
H5A30_RS07010 1552101..1553087 + 987 WP_040033638.1 quinone oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T169535 WP_039278477.1 NZ_CP059957:1548256-1548672 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T169535 NZ_CP076589:848413-848832 [Erwinia amylovora]
GTGGTCCTGTGGCAATGTGAACTGCGGCCATCAAGGCTGGCACAGCGGTTTTCGCTGCTGCTGCATGGGGCAGTGATGCT
GGCGCTGCTATTGCCGACATGGCCCGCCAGCTCTGGCCTGGTGCGGATGCTGTTGCTGGTTTTGGTACTGTTGGAGTGCA
TACGCAGTCGGCGGCGCATTGGTCGTCGCCAGGGGGATATCGCTCTGTTGGAAGGGCATGAGCTACGCTGGCGTCAACGC
GAGTGGTGCATTATTTCCCGTCCGTGGCTGACCGGGCAGGCAATCCTGCTGTTACTACAGGATACTCATGGACAGCGCGA
GCGACTATGGCTGTTCGCCGACGGCATGGAAAAACGTAACTGGCGTCAGCTGCGCCTGCAATTGCTGAACAGTAAAGTAC
AGGGGAATGGCTGGTGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT169535 WP_039278475.1 NZ_CP059957:1548032-1548259 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT169535 NZ_CP076589:848166-848432 [Erwinia amylovora]
ATGGATATTACAAATAAATCACGTATTCACTGGGCATGCCGTCGCGGCATGCGCGAGCTGGATATCTCGATCATGCCCTT
TTTCCAGTATGAATTTGACTCGCTGAACGACAGCGATAAACGGCTGTTCGTTCGCCTGCTGGAGAGCGATGACCCGGACC
TGTTCAACTGGCTGATGAACCACGGCGAGCCGGCAGACGTGGAGCTGAAAAGGATGGTGGCGCTAATCCAACAAAGAAAT
AAACAACGTGGTCCTGTGGCAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References