Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 900686..901303 Replicon chromosome
Accession NZ_CP059957
Organism Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A30_RS04130 Protein ID WP_010281507.1
Coordinates 901124..901303 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A30_RS04125 Protein ID WP_014916995.1
Coordinates 900686..901096 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A30_RS04110 896550..897593 - 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -
H5A30_RS04115 897902..900100 - 2199 WP_104209601.1 primosomal protein N' -
H5A30_RS04120 900374..900589 + 216 WP_010281510.1 50S ribosomal protein L31 -
H5A30_RS04125 900686..901096 - 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A30_RS04130 901124..901303 - 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A30_RS04135 901387..902397 - 1011 WP_119870817.1 iron ABC transporter permease -
H5A30_RS04140 902394..903356 - 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A30_RS04145 903366..904163 - 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A30_RS04150 904397..904714 - 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A30_RS04155 904902..906062 + 1161 WP_039465321.1 cystathionine gamma-synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169534 WP_010281507.1 NZ_CP059957:c901303-901124 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169534 NZ_CP076589:594504-594779 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169534 WP_014916995.1 NZ_CP059957:c901096-900686 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169534 NZ_CP076589:594252-594500 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References