Detailed information of TA system TA169533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 876872..877506
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5A30_RS04000
• Protein ID: WP_014917023.1
• Coordinates: 876872..877147 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: H5A30_RS04005
• Protein ID: WP_014917022.1
• Coordinates: 877144..877506 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS03985	873892..875262	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
H5A30_RS03990	875259..875957	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
H5A30_RS03995	876111..876635	+	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
H5A30_RS04000	876872..877147	+	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5A30_RS04005	877144..877506	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5A30_RS04010	877880..879067	+	1188	WP_080162913.1	sugar transporter	-
H5A30_RS04015	879140..880042	+	903	WP_119870811.1	CDF family cation-efflux transporter FieF	-
H5A30_RS04020	880250..881212	+	963	WP_010281567.1	6-phosphofructokinase	-
H5A30_RS04025	881493..882377	+	885	WP_014917008.1	N-carbamoylputrescine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T169533 WP_014917023.1 NZ_CP059957:876872-877147 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T169533 NZ_CP076589:592889-593209 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT169533 WP_014917022.1 NZ_CP059957:877144-877506 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT169533 NZ_CP076589:592529-592852 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7