Detailed information of TA system TA169530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 710888..712476
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: H5A30_RS03295
• Protein ID: WP_119870763.1
• Coordinates: 711157..712476 (+)
• Length: 440 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H5A30_RS03290
• Protein ID: WP_103943261.1
• Coordinates: 710888..711157 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS03260	705926..706585	+	660	WP_014917137.1	DUF799 domain-containing protein	-
H5A30_RS03265	706708..708210	-	1503	WP_119870760.1	MFS transporter	-
H5A30_RS03270	708314..708865	+	552	WP_119870761.1	TetR/AcrR family transcriptional regulator	-
H5A30_RS03275	708896..709306	-	411	WP_094431545.1	helix-turn-helix transcriptional regulator	-
H5A30_RS03280	709449..710300	+	852	WP_119870762.1	SDR family oxidoreductase	-
H5A30_RS03285	710429..710695	+	267	Protein_635	alpha/beta hydrolase	-
H5A30_RS03290	710888..711157	+	270	WP_103943261.1	helix-turn-helix domain-containing protein	Antitoxin
H5A30_RS03295	711157..712476	+	1320	WP_119870763.1	type II toxin-antitoxin system HipA family toxin	Toxin
H5A30_RS03300	712509..713873	-	1365	WP_119870764.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
H5A30_RS03305	714003..715637	-	1635	WP_010281240.1	membrane protein insertase YidC	-
H5A30_RS03310	715640..715897	-	258	WP_010281237.1	membrane protein insertion efficiency factor YidD	-
H5A30_RS03315	715861..716220	-	360	WP_010281235.1	ribonuclease P protein component	-
H5A30_RS03320	716239..716379	-	141	WP_005976668.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 440 a.a.
• Molecular weight: 49397.67 Da
• Isoelectric Point: 6.6897

>T169530 WP_119870763.1 NZ_CP059957:711157-712476 [Pectobacterium brasiliense]
MAALDVYMNGYRVGILTKTGSGAHHFSYDTNWLGLLGSRPISLSMPLRHQPYQGDEVYNFFDNLLPDNPDIRRRIVARHH
TDSTQPFDLLAKVGQDSVGALQLVPQGTPVHDIKKIEYKTLSEQQLENILTGYLSDAPLGMIDTEDDFRISIAGAQEKTA
LLYLDDRWCLPLNVTPTTHIIKLPIGKIESHSYSIDMSDSVENEYLCLLIAKAFGLPVPHCFMINAGKIKALAVERFDRK
YASDVSWIMRLPQEDFCQVLNVPSALKYENHGGPGISAIMSFLLGSVYPERDRYRFMQTQVLFWLLAATDGHAKNFSLFI
ESEGRYRLTPFYDILSMYPAMGGRGIDRREAKLAMGLTGSRGKKYAIEQIFPRHFFQTAKVVGFARESMENILAEFAQTV
DTVMTTVGSQLPVDFPTHISEAILAGLQARSRRLMSGWE

Nucleotide

• Length: 1320 bp

>T169530 NZ_CP076554:c86423-86007 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10062.40 Da
• Isoelectric Point: 9.7281

>AT169530 WP_103943261.1 NZ_CP059957:710888-711157 [Pectobacterium brasiliense]
MMKITNSKQLSSYLKDVRITQKLSQGKVASKVGIRQDTVSSFEQHPDSTKLETFFKILSALNLELTVTPRNVDTTNNEAS
VASSWKEEW

Nucleotide

• Length: 270 bp

>AT169530 NZ_CP076554:c86650-86420 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA