Detailed information of TA system TA169526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 161268..161795
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H5A30_RS00740
• Protein ID: WP_119871082.1
• Coordinates: 161268..161543 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H5A30_RS00745
• Protein ID: WP_119871081.1
• Coordinates: 161547..161795 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS00715	156426..157658	+	1233	WP_119871085.1	FtsX-like permease family protein	-
H5A30_RS00720	157648..159183	+	1536	WP_119871084.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
H5A30_RS00725	159206..159910	+	705	WP_014913963.1	hypothetical protein	-
H5A30_RS00730	159921..160403	+	483	WP_014913962.1	hypothetical protein	-
H5A30_RS00735	160708..161220	-	513	WP_119871083.1	AAA family ATPase	-
H5A30_RS00740	161268..161543	-	276	WP_119871082.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5A30_RS00745	161547..161795	-	249	WP_119871081.1	ribbon-helix-helix domain-containing protein	Antitoxin
H5A30_RS00750	161905..162090	-	186	Protein_148	DUF4942 domain-containing protein	-
H5A30_RS00755	162109..165357	-	3249	WP_119871080.1	type I restriction endonuclease subunit R	-
H5A30_RS00760	165354..166703	-	1350	WP_199679142.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	160708..175961	15253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11046.26 Da
• Isoelectric Point: 5.1631

>T169526 WP_119871082.1 NZ_CP059957:c161543-161268 [Pectobacterium brasiliense]
MEIRWTQKAQDDLERIYGFASQYSRQHADEVLDRLIIGTTGLADYPRIGVSQTRYEPREVRKILFDDYEVHYEIQHDTSY
IVDLWHKREDR

Nucleotide

• Length: 276 bp

>T169526 NZ_CP076547:c2995765-2995328 [Subtercola sp. PAMC28395]
GTGATTCCACAGCCACGTTACGCGCTCGACACAAGTGTCTCTGTGCCGCTCCTGATGAAGGCACACGACGCTCACGACGT
CGTCTCTGCGTGGGCCGCTGGCCGAGTGCTTGGACTCAGTGGACACGCACTCGTCGAGACCTACTCGGTTCTGACTCGCC
TCCCGGGAGATTCTCGGGTCGATTCCGCTGACGCCGTATTGCTCATAGACGACAACTTCCCCGCCCATTTCGCGCTCTCC
GCAGAAGCCACGAGCACGTCTCATCGCGAACTCGCGCGGCACGGCGTTTCAGGTGGAGCAACATACGACGGTCTCGTCGC
CCTGGCGGCCCGGGAACACGAAGCGGTGTTGGTGACGCGTGACGCGCGGGCCAGACAGACCTATGAGGCCCTGGGCGTCA
AGGTGGAGATCATCACCGTCCCGAGCGTGTCACGGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9090.52 Da
• Isoelectric Point: 10.0121

>AT169526 WP_119871081.1 NZ_CP059957:c161795-161547 [Pectobacterium brasiliense]
MGQPAKQTISAQIPAELAAAVERLAIELDRSKSWVIKEALTAMIEERERRHQRILKGLADVDAGRVVSHADVIDFANKLK
KS

Nucleotide

• Length: 249 bp

>AT169526 NZ_CP076547:c2996001-2995762 [Subtercola sp. PAMC28395]
ATGGAAGCGACGATTGACTCTGGCGGCAGACTCCTTCTACCGAAGAGCCTTCGTGATGCATTGGGTCTTGGCCCGGGTTC
GAAAGTAGACGTGTCGGCCTATGGTGGAGGGCTGCAGGTGACGCCGGGCGGGCGGACTGCGCGCTTGGAGCGGGGTGAAG
ATGGGCGTCTGGTGGCAAGTTCAGAGACATCTGTCTCAGACGACGTTCTGTTCGCACTGATCGATTCTGGGCGGAAGTGA