Detailed information of TA system TA169524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 35576..36360
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5A30_RS00205
• Protein ID: WP_119871144.1
• Coordinates: 35576..36070 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: H5A30_RS00210
• Protein ID: WP_038921611.1
• Coordinates: 36067..36360 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS00180	30974..31716	+	743	Protein_35	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A30_RS00185	31775..31993	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
H5A30_RS00190	31996..32301	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
H5A30_RS00195	32501..33343	-	843	WP_119871146.1	DNA adenine methylase	-
H5A30_RS00200	33429..35150	-	1722	WP_119871145.1	AAA family ATPase	-
H5A30_RS00205	35576..36070	-	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
H5A30_RS00210	36067..36360	-	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
H5A30_RS00215	36700..36942	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A30_RS00220	37046..37300	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A30_RS00225	37385..37633	+	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
H5A30_RS00230	38286..40040	-	1755	WP_119871142.1	AIPR family protein	-
H5A30_RS00235	40268..41053	-	786	WP_185893700.1	phosphonate metabolism protein PhnP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1372..36942	35570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T169524 WP_119871144.1 NZ_CP059957:c36070-35576 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T169524 NZ_CP076545:122024-122302 [Collinsella sp. zg1085]
ATGGCACATTACGAAATAAAGTTCTCCGCCCTGTTTCGCAAACAGCTTAAAGCTGCAAAAAAGCGCGGCAAGGTTCTCAC
TAAGCTGCAAAAAATTATTGACCTGCTTGCTGATGATACAATGCTGCCTGCCAAATATAGAGACCACGCGCTCGAAGGTA
GATGGGTTAATCATAGAGAATGCCATATTGAACCAGATTGGCTGCTCATCTATTACAAACAAGAGGATGTTCTCGTCCTT
ACCTTAGTGGCGACAGGAAGTCATTCTGACCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT169524 WP_038921611.1 NZ_CP059957:c36360-36067 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169524 NZ_CP076545:121743-122024 [Collinsella sp. zg1085]
ATGGCTACAACTAATATTAGTATTCGTATGGAAGAGAGCTTGAAAAAGGAAGCTGATCAATTGTTCAATGAAATGGGAAT
GAATATTTCTACAGCATTTAATATCTTTGTACGTCGAGCGATTCGTGAACGTGCGATTCCTTTTCATATTAGCCTAGGCG
AGCTCAACCATGAGACTATTGCTGCTATGCTAGAAGCAGAGCGCATTGCAAAAGATCCAAACATTCCAAGCTACGTTACA
GCTAAAGAAGCATTGGCAGCGGCACTTTGTGAAGACAACTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3