Detailed information of TA system TA169523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 31775..32301
• Replicon: chromosome
• Accession: NZ_CP059957
• Organism: Pectobacterium brasiliense strain IPO:4071 NAK:240

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A30_RS00190
• Protein ID: WP_119871147.1
• Coordinates: 31996..32301 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: H5A30_RS00185
• Protein ID: WP_015855076.1
• Coordinates: 31775..31993 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A30_RS00155	26802..27689	-	888	WP_109491861.1	ParA family protein	-
H5A30_RS00160	27978..29240	+	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-
H5A30_RS00165	29356..29550	-	195	WP_044209559.1	DUF3950 domain-containing protein	-
H5A30_RS00170	29736..30077	+	342	WP_119871225.1	hypothetical protein	-
H5A30_RS00175	30531..30881	-	351	WP_158586005.1	hypothetical protein	-
H5A30_RS00180	30974..31716	+	743	Protein_35	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A30_RS00185	31775..31993	+	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H5A30_RS00190	31996..32301	+	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
H5A30_RS00195	32501..33343	-	843	WP_119871146.1	DNA adenine methylase	-
H5A30_RS00200	33429..35150	-	1722	WP_119871145.1	AAA family ATPase	-
H5A30_RS00205	35576..36070	-	495	WP_119871144.1	GNAT family N-acetyltransferase	-
H5A30_RS00210	36067..36360	-	294	WP_038921611.1	DUF1778 domain-containing protein	-
H5A30_RS00215	36700..36942	-	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A30_RS00220	37046..37300	+	255	WP_119871224.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1372..36942	35570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T169523 WP_119871147.1 NZ_CP059957:31996-32301 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T169523 NZ_CP076539:2433705-2434442 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACCACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAAGAATATACAAAGAGCTTAAATGTATTTTTAGATTCATATGGGGAAAAAATTGGCGCGAAAATT
GTTGTTTTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGGATCATACCAGCAAAAATAGTAAGTGATG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT169523 WP_015855076.1 NZ_CP059957:31775-31993 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT169523 NZ_CP076539:2434555-2434683 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATGC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46