Detailed information of TA system TA169516

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1480019..1480743
• Replicon: chromosome
• Accession: NZ_CP059956
• Organism: Pectobacterium brasiliense strain IPO:4132 NAK:239

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: H5A38_RS06550
• Protein ID: WP_205546782.1
• Coordinates: 1480019..1480339 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: H5A38_RS06555
• Protein ID: WP_205546781.1
• Coordinates: 1480408..1480743 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A38_RS06525	1475479..1477089	+	1611	WP_205546786.1	hypothetical protein	-
H5A38_RS06530	1477390..1477627	-	238	Protein_1263	IS110 family transposase	-
H5A38_RS06535	1478339..1478743	-	405	WP_205546785.1	tRNA(fMet)-specific endonuclease VapC	-
H5A38_RS06540	1478743..1478973	-	231	WP_205546784.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H5A38_RS06545	1479068..1479901	-	834	WP_205546783.1	DUF4942 domain-containing protein	-
H5A38_RS06550	1480019..1480339	-	321	WP_205546782.1	TA system toxin CbtA family protein	Toxin
H5A38_RS06555	1480408..1480743	-	336	WP_205546781.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H5A38_RS06560	1480783..1481256	-	474	WP_205546780.1	DNA repair protein RadC	-
H5A38_RS06565	1481341..1481574	-	234	WP_198481703.1	hypothetical protein	-
H5A38_RS06570	1481591..1482064	-	474	WP_205546779.1	hypothetical protein	-
H5A38_RS06575	1482100..1482669	-	570	WP_025919489.1	hypothetical protein	-
H5A38_RS06580	1482851..1483549	-	699	Protein_1273	DeoR family transcriptional regulator	-
H5A38_RS06585	1483775..1484596	-	822	WP_205546778.1	DUF932 domain-containing protein	-
H5A38_RS06590	1484681..1485556	-	876	WP_205546777.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-105)

Antitoxin

• Antitoxin CbeA (7-106)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12140.88 Da
• Isoelectric Point: 4.5971

>T169516 WP_205546782.1 NZ_CP059956:c1480339-1480019 [Pectobacterium brasiliense]
MQTSPAIPPREDNPCPSPIAVWQQLLTYLLEKHYGLTLNDTPFCEENVIQEHIDAGITLVNAMNFLVEKYELVRIDRDGF
NWQEQSPFLTTVDILRARRATGLLKA

Nucleotide

• Length: 321 bp

>T169516 NZ_CP076531:c29293-29141 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12456.25 Da
• Isoelectric Point: 6.4457

>AT169516 WP_205546781.1 NZ_CP059956:c1480743-1480408 [Pectobacterium brasiliense]
MPSADTPEWGLKRAVTPRFGARLVQEGNRLHYLADRASIVGTFSKTEAQSLERCFPELIKLLEQKLRMGELNPRQQNCVT
LHCNEWICEADTLGSVGYVYIVIYPSSAAAE

Nucleotide

• Length: 336 bp

>AT169516 NZ_CP076531:29345-29405 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT