Detailed information of TA system TA169514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 886645..887285
• Replicon: chromosome
• Accession: NZ_CP059956
• Organism: Pectobacterium brasiliense strain IPO:4132 NAK:239

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A7V8Q162
• Locus tag: H5A38_RS03940
• Protein ID: WP_039278477.1
• Coordinates: 886869..887285 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: H5A38_RS03935
• Protein ID: WP_205545905.1
• Coordinates: 886645..886872 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A38_RS03905	881773..882177	+	405	WP_205547287.1	DUF1240 domain-containing protein	-
H5A38_RS03910	882229..882465	-	237	Protein_763	type VI secretion system tube protein Hcp	-
H5A38_RS03915	882847..883344	-	498	WP_039278469.1	M48 family metallopeptidase	-
H5A38_RS03920	883428..884621	-	1194	WP_039465897.1	aspartate/tyrosine/aromatic aminotransferase	-
H5A38_RS03925	884685..885299	-	615	WP_010279239.1	YitT family protein	-
H5A38_RS03930	885550..886029	-	480	WP_010295086.1	Lrp/AsnC family transcriptional regulator	-
H5A38_RS03935	886645..886872	+	228	WP_205545905.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H5A38_RS03940	886869..887285	+	417	WP_039278477.1	type II toxin-antitoxin system VapC family toxin	Toxin
H5A38_RS03945	887425..888063	-	639	WP_205545906.1	hypothetical protein	-
H5A38_RS03950	888056..889138	-	1083	WP_203449520.1	alanine racemase	-
H5A38_RS03955	889180..890586	-	1407	WP_010279223.1	replicative DNA helicase	-
H5A38_RS03960	890909..891895	+	987	WP_205545907.1	NADPH:quinone reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15274.80 Da
• Isoelectric Point: 8.2931

>T169514 WP_039278477.1 NZ_CP059956:886869-887285 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Nucleotide

• Length: 417 bp

>T169514 NZ_CP076529:52612-53028 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8906.15 Da
• Isoelectric Point: 5.1532

>AT169514 WP_205545905.1 NZ_CP059956:886645-886872 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMKERQDVVEEGRFKL

Nucleotide

• Length: 228 bp

>AT169514 NZ_CP076529:52385-52615 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7V8Q162