Detailed information of TA system TA169513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 199886..200520
• Replicon: chromosome
• Accession: NZ_CP059956
• Organism: Pectobacterium brasiliense strain IPO:4132 NAK:239

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5A38_RS00880
• Protein ID: WP_080162911.1
• Coordinates: 199886..200161 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: H5A38_RS00885
• Protein ID: WP_014917022.1
• Coordinates: 200158..200520 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A38_RS00865	196906..198276	-	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-
H5A38_RS00870	198273..198971	-	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
H5A38_RS00875	199125..199649	+	525	WP_205547168.1	cell-envelope stress modulator CpxP	-
H5A38_RS00880	199886..200161	+	276	WP_080162911.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5A38_RS00885	200158..200520	+	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5A38_RS00890	200894..202081	+	1188	WP_014917021.1	sugar transporter	-
H5A38_RS00895	202145..203047	+	903	WP_039281625.1	CDF family cation-efflux transporter FieF	-
H5A38_RS00900	203255..204217	+	963	WP_010306854.1	6-phosphofructokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10024.61 Da
• Isoelectric Point: 11.1875

>T169513 WP_080162911.1 NZ_CP059956:199886-200161 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLSQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T169513 NZ_CP076529:c46211-45906 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT169513 WP_014917022.1 NZ_CP059956:200158-200520 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT169513 NZ_CP076529:c46431-46213 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7