Detailed information of TA system TA169508

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2123333..2123993
• Replicon: chromosome
• Accession: NZ_CP059955
• Organism: Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: H5A16_RS09695
• Protein ID: WP_072061666.1
• Coordinates: 2123580..2123993 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A1D7Z1Z6
• Locus tag: H5A16_RS09690
• Protein ID: WP_005971623.1
• Coordinates: 2123333..2123599 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A16_RS09670	2119296..2120300	-	1005	WP_119870587.1	LacI family DNA-binding transcriptional regulator	-
H5A16_RS09675	2120355..2120963	-	609	WP_010279828.1	HD domain-containing protein	-
H5A16_RS09680	2121394..2122041	+	648	WP_010279833.1	hemolysin III family protein	-
H5A16_RS09685	2122096..2123097	-	1002	WP_010279836.1	tRNA-modifying protein YgfZ	-
H5A16_RS09690	2123333..2123599	+	267	WP_005971623.1	FAD assembly factor SdhE	Antitoxin
H5A16_RS09695	2123580..2123993	+	414	WP_072061666.1	protein YgfX	Toxin
H5A16_RS09700	2124062..2124997	+	936	WP_119870588.1	23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF	-
H5A16_RS09705	2125063..2125386	-	324	WP_119870589.1	hypothetical protein	-
H5A16_RS09710	2125415..2125933	-	519	WP_119870590.1	flavodoxin FldB	-
H5A16_RS09715	2126276..2126662	-	387	WP_048256533.1	acyl-CoA thioesterase	-
H5A16_RS09720	2126867..2128195	-	1329	WP_039282120.1	MHS family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16325.29 Da
• Isoelectric Point: 10.7911

>T169508 WP_072061666.1 NZ_CP059955:2123580-2123993 [Pectobacterium brasiliense]
VAQWRCDLRVSWRMQLLSLVVHGLLVLLILLAPWPDGYAWLWLCLVTMVMFGFIRSQRNIKSRQGEIVLLSETTLNWRQQ
EWQIVKRPWLLKNGILLSLQAVNGKDKQQLWLASDSMGDDEWRHLRQILLQQKHWAR

Nucleotide

• Length: 414 bp

>T169508 NZ_CP076527:3880914-3881321 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10507.94 Da
• Isoelectric Point: 5.1392

>AT169508 WP_005971623.1 NZ_CP059955:2123333-2123599 [Pectobacterium brasiliense]
MEIDNKSRIHWACRRGMRELDIAIMPFFEHDYDTLNDTDKRTFVRLLQSDDPDLFNWLMNHGEPEDGELKRMISLIQTRN
KDRGPVAM

Nucleotide

• Length: 267 bp

>AT169508 NZ_CP076527:3880667-3880933 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1D7Z1Z6