Detailed information of TA system TA169507

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1941187..1941713
• Replicon: chromosome
• Accession: NZ_CP059955
• Organism: Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H5A16_RS08755
• Protein ID: WP_119871147.1
• Coordinates: 1941187..1941492 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: C6C380
• Locus tag: H5A16_RS08760
• Protein ID: WP_015855076.1
• Coordinates: 1941495..1941713 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A16_RS08725	1936188..1936442	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A16_RS08730	1936546..1936788	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A16_RS08735	1937128..1937421	+	294	WP_038921611.1	DUF1778 domain-containing protein	-
H5A16_RS08740	1937418..1937912	+	495	WP_119871144.1	GNAT family N-acetyltransferase	-
H5A16_RS08745	1938338..1940059	+	1722	WP_119871145.1	AAA family ATPase	-
H5A16_RS08750	1940145..1940987	+	843	WP_119871146.1	DNA adenine methylase	-
H5A16_RS08755	1941187..1941492	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	Toxin
H5A16_RS08760	1941495..1941713	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H5A16_RS08765	1941772..1942515	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
H5A16_RS08770	1942608..1942958	+	351	WP_158586005.1	hypothetical protein	-
H5A16_RS08775	1943412..1943753	-	342	WP_119871225.1	hypothetical protein	-
H5A16_RS08780	1943939..1944133	+	195	WP_044209559.1	DUF3950 domain-containing protein	-
H5A16_RS08785	1944249..1945511	-	1263	WP_119871150.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1936872..1945688	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11759.63 Da
• Isoelectric Point: 4.6533

>T169507 WP_119871147.1 NZ_CP059955:c1941492-1941187 [Pectobacterium brasiliense]
MQFIVYQYKRASHYKMFVDVQSDIVETPKRRMVIPLIEAYHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVT
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T169507 NZ_CP076527:3746797-3747093 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATACCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8178.13 Da
• Isoelectric Point: 7.0135

>AT169507 WP_015855076.1 NZ_CP059955:c1941713-1941495 [Pectobacterium brasiliense]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQNGSFADENRNW

Nucleotide

• Length: 219 bp

>AT169507 NZ_CP076527:3747095-3747490 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A250BG46