Detailed information of TA system TA169506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1937128..1937912
• Replicon: chromosome
• Accession: NZ_CP059955
• Organism: Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: H5A16_RS08740
• Protein ID: WP_119871144.1
• Coordinates: 1937418..1937912 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A3S0Y1Y3
• Locus tag: H5A16_RS08735
• Protein ID: WP_038921611.1
• Coordinates: 1937128..1937421 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A16_RS08710	1932435..1933220	+	786	WP_185893700.1	phosphonate metabolism protein PhnP	-
H5A16_RS08715	1933448..1935202	+	1755	WP_119871142.1	AIPR family protein	-
H5A16_RS08720	1935855..1936103	-	249	WP_119871143.1	helix-turn-helix domain-containing protein	-
H5A16_RS08725	1936188..1936442	-	255	WP_119871224.1	helix-turn-helix domain-containing protein	-
H5A16_RS08730	1936546..1936788	+	243	WP_072010299.1	helix-turn-helix domain-containing protein	-
H5A16_RS08735	1937128..1937421	+	294	WP_038921611.1	DUF1778 domain-containing protein	Antitoxin
H5A16_RS08740	1937418..1937912	+	495	WP_119871144.1	GNAT family N-acetyltransferase	Toxin
H5A16_RS08745	1938338..1940059	+	1722	WP_119871145.1	AAA family ATPase	-
H5A16_RS08750	1940145..1940987	+	843	WP_119871146.1	DNA adenine methylase	-
H5A16_RS08755	1941187..1941492	-	306	WP_119871147.1	type II toxin-antitoxin system toxin CcdB	-
H5A16_RS08760	1941495..1941713	-	219	WP_015855076.1	type II toxin-antitoxin system antitoxin CcdA	-
H5A16_RS08765	1941772..1942515	-	744	WP_119871148.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1936872..1945688	8816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17689.53 Da
• Isoelectric Point: 7.2150

>T169506 WP_119871144.1 NZ_CP059955:1937418-1937912 [Pectobacterium brasiliense]
MISAPEPLRAEHILSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T169506 NZ_CP076527:c3633722-3633258 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10856.44 Da
• Isoelectric Point: 10.1968

>AT169506 WP_038921611.1 NZ_CP059955:1937128-1937421 [Pectobacterium brasiliense]
MPATNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT169506 NZ_CP076527:c3634057-3633722 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S0Y1Y3