Detailed information of TA system TA169497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1095983..1096617
• Replicon: chromosome
• Accession: NZ_CP059955
• Organism: Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H5A16_RS04955
• Protein ID: WP_014917023.1
• Coordinates: 1096342..1096617 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A433N1R7
• Locus tag: H5A16_RS04950
• Protein ID: WP_014917022.1
• Coordinates: 1095983..1096345 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5A16_RS04930	1091112..1091996	-	885	WP_014917008.1	N-carbamoylputrescine amidase	-
H5A16_RS04935	1092277..1093239	-	963	WP_010281567.1	6-phosphofructokinase	-
H5A16_RS04940	1093447..1094349	-	903	WP_119870811.1	CDF family cation-efflux transporter FieF	-
H5A16_RS04945	1094422..1095609	-	1188	WP_080162913.1	sugar transporter	-
H5A16_RS04950	1095983..1096345	-	363	WP_014917022.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H5A16_RS04955	1096342..1096617	-	276	WP_014917023.1	type II toxin-antitoxin system HicA family toxin	Toxin
H5A16_RS04960	1096854..1097378	-	525	WP_014917024.1	cell-envelope stress modulator CpxP	-
H5A16_RS04965	1097532..1098230	+	699	WP_010281574.1	envelope stress response regulator transcription factor CpxR	-
H5A16_RS04970	1098227..1099597	+	1371	WP_010281575.1	envelope stress sensor histidine kinase CpxA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10050.70 Da
• Isoelectric Point: 11.1875

>T169497 WP_014917023.1 NZ_CP059955:c1096617-1096342 [Pectobacterium brasiliense]
MQGKIASLRKKQRETLIQIFKTPVLSGVKWSNIESLVTALGGEIKEGSGSRVRFLLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T169497 NZ_CP076527:296460-296563 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13504.31 Da
• Isoelectric Point: 4.4969

>AT169497 WP_014917022.1 NZ_CP059955:c1096345-1095983 [Pectobacterium brasiliense]
MTKALNTPNTMVVSGQPAIISYVPEMGMFRGKFIGLSGYCDFVADSINGLRSEGEISLREYLEDCQENGIEPYEREEKVK
TFTLRYPESFGERLTIAAAERQISVNTFIVETLSEHLKQA

Nucleotide

• Length: 363 bp

>AT169497 NZ_CP076527:c296601-296330 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A433N1R7