Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1072186..1072803 Replicon chromosome
Accession NZ_CP059955
Organism Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)


Gene name hicA Uniprot ID C6DHN3
Locus tag H5A16_RS04825 Protein ID WP_010281507.1
Coordinates 1072186..1072365 (+) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A086ESS8
Locus tag H5A16_RS04830 Protein ID WP_014916995.1
Coordinates 1072393..1072803 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A16_RS04800 1067427..1068587 - 1161 WP_039465321.1 cystathionine gamma-synthase -
H5A16_RS04805 1068775..1069092 + 318 WP_005973355.1 met regulon transcriptional regulator MetJ -
H5A16_RS04810 1069326..1070123 + 798 WP_119870818.1 ABC transporter ATP-binding protein -
H5A16_RS04815 1070133..1071095 + 963 WP_014916993.1 ABC transporter substrate-binding protein -
H5A16_RS04820 1071092..1072102 + 1011 WP_119870817.1 iron ABC transporter permease -
H5A16_RS04825 1072186..1072365 + 180 WP_010281507.1 type II toxin-antitoxin system HicA family toxin Toxin
H5A16_RS04830 1072393..1072803 + 411 WP_014916995.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H5A16_RS04835 1072900..1073115 - 216 WP_010281510.1 50S ribosomal protein L31 -
H5A16_RS04840 1073389..1075587 + 2199 WP_104209601.1 primosomal protein N' -
H5A16_RS04845 1075896..1076939 + 1044 WP_010281514.1 DNA-binding transcriptional regulator CytR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6560.69 Da        Isoelectric Point: 10.7838

>T169496 WP_010281507.1 NZ_CP059955:1072186-1072365 [Pectobacterium brasiliense]
MDSRTLIAEIKADGWELIRVNGSHHHFMHPSKPGLVTIPHPKKDLPIGTVKSIRKQAGI

Download         Length: 180 bp

>T169496 NZ_CP076520:65703-65984 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14506.39 Da        Isoelectric Point: 4.3391

>AT169496 WP_014916995.1 NZ_CP059955:1072393-1072803 [Pectobacterium brasiliense]
MFYPIAIEAGDDTHAYGVTVPDLPGCFSAGDTLDDAIANAKEAITGHIELLIEMGQDIPTVSSVGQLAKSAEYAGYTWAV
VDIDVTRLMGGSEKINVTLPKSLIDRIDRCVASNPEFKSRSGFLAQAALERISSSR

Download         Length: 411 bp

>AT169496 NZ_CP076520:65462-65713 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W2I1S0

Antitoxin

Source ID Structure
AlphaFold DB A0A086ESS8

References