Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 424814..425454 Replicon chromosome
Accession NZ_CP059955
Organism Pectobacterium brasiliense strain IPO:4134 NAK:395

Toxin (Protein)


Gene name vagD Uniprot ID A0A7V8Q162
Locus tag H5A16_RS01960 Protein ID WP_039278477.1
Coordinates 424814..425230 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A086EF97
Locus tag H5A16_RS01965 Protein ID WP_039278475.1
Coordinates 425227..425454 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5A16_RS01940 420399..421385 - 987 WP_040033638.1 quinone oxidoreductase -
H5A16_RS01945 421514..422920 + 1407 WP_010279223.1 replicative DNA helicase -
H5A16_RS01950 422962..424044 + 1083 WP_119872014.1 alanine racemase -
H5A16_RS01955 424037..424675 + 639 WP_039517591.1 hypothetical protein -
H5A16_RS01960 424814..425230 - 417 WP_039278477.1 type II toxin-antitoxin system VapC family toxin Toxin
H5A16_RS01965 425227..425454 - 228 WP_039278475.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5A16_RS01970 426371..426850 + 480 WP_010295086.1 Lrp/AsnC family transcriptional regulator -
H5A16_RS01975 427101..427715 + 615 WP_119872013.1 YitT family protein -
H5A16_RS01980 427779..428972 + 1194 WP_119872012.1 aspartate/tyrosine/aromatic aminotransferase -
H5A16_RS01985 429056..429553 + 498 WP_039278469.1 M48 family metallopeptidase -
H5A16_RS01990 429678..430157 + 480 WP_014916572.1 Hcp family type VI secretion system effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-55)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15274.80 Da        Isoelectric Point: 8.2931

>T169495 WP_039278477.1 NZ_CP059955:c425230-424814 [Pectobacterium brasiliense]
VSRLFMLDTNICSFIMREHPAEVIEKLQQCTLRRDRIVVSAITYAEMRFGAVGKKASPRHMQLVDAFCRRLDAVLPWDSA
AVDATTKIKIELAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWVK

Download         Length: 417 bp

>T169495 NZ_CP076519:105098-105580 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8879.08 Da        Isoelectric Point: 4.8359

>AT169495 WP_039278475.1 NZ_CP059955:c425454-425227 [Pectobacterium brasiliense]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIMLRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFKL

Download         Length: 228 bp

>AT169495 NZ_CP076519:104844-105110 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V8Q162

Antitoxin

Source ID Structure
AlphaFold DB A0A086EF97

References