Detailed information of TA system TA169494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 17617..18139
• Replicon: plasmid p2
• Accession: NZ_CP059949
• Organism: Escherichia coli strain 268.2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: H5C70_RS24280
• Protein ID: WP_000220560.1
• Coordinates: 17617..17898 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: H5C70_RS24285
• Protein ID: WP_000121743.1
• Coordinates: 17888..18139 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C70_RS24230	13469..14635	-	1167	WP_079905805.1	relaxase/mobilization nuclease domain-containing protein	-
H5C70_RS24235	14638..15183	-	546	WP_000757693.1	hypothetical protein	-
H5C70_RS24240	15178..15252	-	75	Protein_16	DNA distortion polypeptide 3	-
H5C70_RS24245	15505..15777	-	273	WP_000160399.1	hypothetical protein	-
H5C70_RS24250	15790..15939	-	150	WP_000003880.1	hypothetical protein	-
H5C70_RS24255	16009..16188	-	180	WP_000439078.1	hypothetical protein	-
H5C70_RS24260	16231..16560	-	330	WP_000866648.1	hypothetical protein	-
H5C70_RS24265	16653..16868	-	216	WP_001180116.1	hypothetical protein	-
H5C70_RS24270	16858..17103	-	246	WP_000356546.1	hypothetical protein	-
H5C70_RS24275	17148..17471	-	324	WP_001181903.1	hypothetical protein	-
H5C70_RS24280	17617..17898	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5C70_RS24285	17888..18139	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
H5C70_RS24290	19373..20212	+	840	WP_021526660.1	replication initiation protein	-
H5C70_RS24295	20233..21075	+	843	WP_000435061.1	replication initiation protein	-
H5C70_RS24300	21079..21522	+	444	WP_001074380.1	hypothetical protein	-
H5C70_RS24305	21593..22018	+	426	WP_000202954.1	hypothetical protein	-
H5C70_RS24310	22015..22992	-	978	WP_001269912.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37662	37662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T169494 WP_000220560.1 NZ_CP059949:c17898-17617 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T169494 NZ_CP076519:57487-57903 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT169494 WP_000121743.1 NZ_CP059949:c18139-17888 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT169494 NZ_CP076519:57260-57490 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301