169493

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 116608..117251 Replicon plasmid p1
Accession NZ_CP059948
Organism Escherichia coli strain 268.2

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H5C70_RS24055 Protein ID WP_001034044.1
Coordinates 116835..117251 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H5C70_RS24050 Protein ID WP_001261286.1
Coordinates 116608..116838 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C70_RS24030 113239..113994 - 756 WP_000852146.1 replication initiation protein RepE -
H5C70_RS24035 114716..115522 - 807 WP_000016970.1 site-specific integrase -
H5C70_RS24040 115523..115828 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H5C70_RS24045 115830..116048 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H5C70_RS24050 116608..116838 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5C70_RS24055 116835..117251 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H5C70_RS24060 117326..118891 + 1566 WP_001128474.1 AAA family ATPase -
H5C70_RS24065 118876..119898 + 1023 WP_000361402.1 helicase UvrD -
H5C70_RS24070 120152..120849 - 698 Protein_140 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IId / qnrS1 / blaCTX-M-55 / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..138915 138915
- flank IS/Tn - - 120152..120529 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T169493 WP_001034044.1 NZ_CP059948:116835-117251 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T169493 NZ_CP076518:c5245117-5244734 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT169493 WP_001261286.1 NZ_CP059948:116608-116838 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT169493 NZ_CP076518:c5245359-5245117 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References