169475

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3530385..3531222 Replicon chromosome
Accession NZ_CP059947
Organism Escherichia coli strain 268.2

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H5C70_RS17040 Protein ID WP_000227784.1
Coordinates 3530680..3531222 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H5C70_RS17035 Protein ID WP_001297137.1
Coordinates 3530385..3530696 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C70_RS17010 3525405..3526352 + 948 WP_021548571.1 cytochrome o ubiquinol oxidase subunit II -
H5C70_RS17015 3526374..3528365 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H5C70_RS17020 3528355..3528969 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H5C70_RS17025 3528969..3529298 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H5C70_RS17030 3529310..3530200 + 891 WP_000971336.1 heme o synthase -
H5C70_RS17035 3530385..3530696 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H5C70_RS17040 3530680..3531222 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H5C70_RS17045 3531278..3532213 - 936 WP_001297127.1 sel1 repeat family protein -
H5C70_RS17050 3532621..3533985 + 1365 WP_001000978.1 MFS transporter -
H5C70_RS17055 3534113..3534604 - 492 WP_001138904.1 nucleotide binding protein YajQ -
H5C70_RS17060 3534772..3535683 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T169475 WP_000227784.1 NZ_CP059947:3530680-3531222 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T169475 NZ_CP076500:41164-41274 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT169475 WP_001297137.1 NZ_CP059947:3530385-3530696 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT169475 NZ_CP076500:c41378-41314 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References