Detailed information of TA system TA169460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1355280..1355905
• Replicon: chromosome
• Accession: NZ_CP059947
• Organism: Escherichia coli strain 268.2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H5C70_RS06610
• Protein ID: WP_000911330.1
• Coordinates: 1355507..1355905 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H5C70_RS06605
• Protein ID: WP_000450524.1
• Coordinates: 1355280..1355507 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C70_RS06580	1351083..1351553	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H5C70_RS06585	1351553..1352125	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H5C70_RS06590	1352271..1353149	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H5C70_RS06595	1353166..1354200	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H5C70_RS06600	1354413..1355126	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H5C70_RS06605	1355280..1355507	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H5C70_RS06610	1355507..1355905	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H5C70_RS06615	1356052..1356915	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
H5C70_RS06620	1356930..1358945	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
H5C70_RS06625	1359019..1359717	+	699	WP_000679823.1	esterase	-
H5C70_RS06630	1359827..1360027	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T169460 WP_000911330.1 NZ_CP059947:1355507-1355905 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T169460 NZ_CP076494:c41397-41122 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT169460 WP_000450524.1 NZ_CP059947:1355280-1355507 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT169460 NZ_CP076494:c41656-41390 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33