169459

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1017985..1018568 Replicon chromosome
Accession NZ_CP059947
Organism Escherichia coli strain 268.2

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag H5C70_RS04920 Protein ID WP_000254745.1
Coordinates 1018233..1018568 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag H5C70_RS04915 Protein ID WP_000581937.1
Coordinates 1017985..1018233 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C70_RS04905 1014324..1015625 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
H5C70_RS04910 1015673..1017907 + 2235 WP_000226815.1 GTP pyrophosphokinase -
H5C70_RS04915 1017985..1018233 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
H5C70_RS04920 1018233..1018568 + 336 WP_000254745.1 endoribonuclease MazF Toxin
H5C70_RS04925 1018639..1019430 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
H5C70_RS04930 1019658..1021295 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
H5C70_RS04935 1021383..1022681 + 1299 WP_000036723.1 phosphopyruvate hydratase -
H5C70_RS04940 1022737..1023099 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T169459 WP_000254745.1 NZ_CP059947:1018233-1018568 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T169459 NZ_CP076494:c629-519 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT169459 WP_000581937.1 NZ_CP059947:1017985-1018233 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT169459 NZ_CP076494:415-479 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References