Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4256718..4257549 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag H5C75_RS20580 Protein ID WP_000854814.1
Coordinates 4256718..4257092 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag H5C75_RS20585 Protein ID WP_001285585.1
Coordinates 4257181..4257549 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS20540 (4252112) 4252112..4253278 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
H5C75_RS20545 (4253397) 4253397..4253870 + 474 WP_032202496.1 DNA gyrase inhibitor SbmC -
H5C75_RS20550 (4254069) 4254069..4255128 + 1060 Protein_4019 FUSC family protein -
H5C75_RS20555 (4255300) 4255300..4255629 + 330 WP_000450409.1 DUF496 family protein -
H5C75_RS20560 (4255730) 4255730..4255864 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
H5C75_RS20565 (4255984) 4255984..4256112 + 129 Protein_4022 transposase domain-containing protein -
H5C75_RS20570 (4256401) 4256401..4256481 - 81 Protein_4023 hypothetical protein -
H5C75_RS20575 (4256527) 4256527..4256721 - 195 WP_000988600.1 DUF5983 family protein -
H5C75_RS20580 (4256718) 4256718..4257092 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
H5C75_RS20585 (4257181) 4257181..4257549 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
H5C75_RS20590 (4257623) 4257623..4257844 - 222 WP_000692323.1 DUF987 domain-containing protein -
H5C75_RS20595 (4257907) 4257907..4258383 - 477 WP_022645117.1 RadC family protein -
H5C75_RS20600 (4258399) 4258399..4258878 - 480 WP_251077434.1 antirestriction protein -
H5C75_RS20605 (4258960) 4258960..4259781 - 822 WP_001234530.1 DUF932 domain-containing protein -
H5C75_RS20610 (4260002) 4260002..4260412 - 411 WP_000846713.1 hypothetical protein -
H5C75_RS20615 (4260428) 4260428..4260805 - 378 Protein_4032 hypothetical protein -
H5C75_RS20620 (4261001) 4261001..4262542 + 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T169445 WP_000854814.1 NZ_CP059929:c4257092-4256718 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T169445 NZ_CP076489:c25793-25398 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCGAATCGCATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGGTTCTGTTGCAAAGTTTTAAATCTACTATCAAATAAGGTAGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT169445 WP_001285585.1 NZ_CP059929:c4257549-4257181 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT169445 NZ_CP076489:c26067-25795 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References