Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ypjF-yeeU/CbtA-CbeA
Location 3580634..3581366 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name ypjF Uniprot ID A0A377CSV0
Locus tag H5C75_RS17470 Protein ID WP_001194227.1
Coordinates 3580634..3580963 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A377CUM4
Locus tag H5C75_RS17475 Protein ID WP_001059335.1
Coordinates 3581001..3581366 (-) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS17465 (3575696) 3575696..3580270 + 4575 WP_247776754.1 adhesin-like autotransporter YpjA/EhaD -
H5C75_RS17470 (3580634) 3580634..3580963 - 330 WP_001194227.1 TA system toxin CbtA family protein Toxin
H5C75_RS17475 (3581001) 3581001..3581366 - 366 WP_001059335.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
H5C75_RS17480 (3581392) 3581392..3581613 - 222 WP_000691984.1 DUF987 domain-containing protein -
H5C75_RS17485 (3581610) 3581610..3582152 - 543 WP_001098974.1 DNA repair protein RadC -
H5C75_RS17490 (3582165) 3582165..3582608 - 444 WP_000381004.1 antirestriction protein -
H5C75_RS17495 (3582639) 3582639..3583460 - 822 WP_001234410.1 DUF932 domain-containing protein -
H5C75_RS17500 (3583552) 3583552..3583893 - 342 WP_000720953.1 hypothetical protein -
H5C75_RS17505 (3583928) 3583928..3584392 - 465 WP_000791791.1 hypothetical protein -
H5C75_RS17510 (3584518) 3584518..3584676 - 159 WP_001128372.1 hypothetical protein -
H5C75_RS17515 (3584898) 3584898..3585785 - 888 WP_001567853.1 50S ribosome-binding GTPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(17-117)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12445.11 Da        Isoelectric Point: 7.2767

>T169443 WP_001194227.1 NZ_CP059929:c3580963-3580634 [Escherichia coli]
MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFSNETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGF
SWQEQTPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T169443 NZ_CP076488:c2763106-2762990 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGTTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13545.40 Da        Isoelectric Point: 5.8806

>AT169443 WP_001059335.1 NZ_CP059929:c3581366-3581001 [Escherichia coli]
MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLADRAGFNGAFIDDNALRLDLAFPLILKQLELMLTSGE
LSPRHEHCVTLYHNGLTCEADTLGSCGYVYIAIYPDQPEPH

Download         Length: 366 bp

>AT169443 NZ_CP076488:2762874-2763079 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAA
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CSV0

Antitoxin

Source ID Structure
AlphaFold DB A0A377CUM4

References