Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3324376..3325030 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H5C75_RS16265 Protein ID WP_000244777.1
Coordinates 3324623..3325030 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H5C75_RS16260 Protein ID WP_000354046.1
Coordinates 3324376..3324642 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS16235 (3319545) 3319545..3320288 + 744 WP_251077407.1 SDR family oxidoreductase -
H5C75_RS16240 (3320345) 3320345..3321778 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
H5C75_RS16245 (3321823) 3321823..3322134 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H5C75_RS16250 (3322298) 3322298..3322957 + 660 WP_000250274.1 hemolysin III family protein -
H5C75_RS16255 (3323153) 3323153..3324133 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H5C75_RS16260 (3324376) 3324376..3324642 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H5C75_RS16265 (3324623) 3324623..3325030 + 408 WP_000244777.1 protein YgfX Toxin
H5C75_RS16270 (3325070) 3325070..3325591 - 522 WP_001055874.1 flavodoxin FldB -
H5C75_RS16275 (3325703) 3325703..3326599 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H5C75_RS16280 (3326624) 3326624..3327334 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H5C75_RS16285 (3327340) 3327340..3329073 + 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T169441 WP_000244777.1 NZ_CP059929:3324623-3325030 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T169441 NZ_CP076488:580407-580508 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT169441 WP_000354046.1 NZ_CP059929:3324376-3324642 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT169441 NZ_CP076488:c580627-580453 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTGAATCTTAACCGTTCGGCCTACGAAGCTTGTGAACGGTTATTTTTTATTGTTTTTCGTTGCTTCAAGGATACCGAAAA
TCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References