Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2026817..2027314 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H5C75_RS10055 Protein ID WP_001173343.1
Coordinates 2027141..2027314 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H5C75_RS10050 Protein ID WP_000398619.1
Coordinates 2026817..2027113 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS10025 (2022212) 2022212..2022931 - 720 WP_000611288.1 two-component system response regulator DcuR -
H5C75_RS10030 (2022928) 2022928..2024559 - 1632 WP_001565376.1 sensor histidine kinase -
H5C75_RS10040 (2026061) 2026061..2026306 + 246 WP_251077526.1 4Fe-4S mono-cluster protein YjdI -
H5C75_RS10045 (2026318) 2026318..2026590 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
H5C75_RS10050 (2026817) 2026817..2027113 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H5C75_RS10055 (2027141) 2027141..2027314 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H5C75_RS10060 (2027433) 2027433..2028950 - 1518 WP_001295074.1 lysine--tRNA ligase -
H5C75_RS10065 (2029187) 2029187..2030644 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T169438 WP_001173343.1 NZ_CP059929:2027141-2027314 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T169438 NZ_CP076488:c408285-408142 [Enterococcus faecalis]
ATGCACGTATTTCCGAAATTCACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT169438 WP_000398619.1 NZ_CP059929:2026817-2027113 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT169438 NZ_CP076488:407954-408209 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTTAAA
AATAACCGTGCTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References