Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1264781..1265399 Replicon chromosome
Accession NZ_CP059929
Organism Escherichia coli strain 162.2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H5C75_RS06385 Protein ID WP_001291435.1
Coordinates 1265181..1265399 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H5C75_RS06380 Protein ID WP_000344800.1
Coordinates 1264781..1265155 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C75_RS06370 (1259869) 1259869..1261062 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H5C75_RS06375 (1261085) 1261085..1264234 + 3150 WP_251077486.1 efflux RND transporter permease AcrB -
H5C75_RS06380 (1264781) 1264781..1265155 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H5C75_RS06385 (1265181) 1265181..1265399 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H5C75_RS06390 (1265571) 1265571..1266122 + 552 WP_000102564.1 maltose O-acetyltransferase -
H5C75_RS06395 (1266238) 1266238..1266708 + 471 WP_000136192.1 YlaC family protein -
H5C75_RS06400 (1266872) 1266872..1268422 + 1551 WP_024238838.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H5C75_RS06405 (1268464) 1268464..1268817 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H5C75_RS06410 (1269161) 1269161..1269388 - 228 WP_024183738.1 DUF2188 domain-containing protein -
H5C75_RS06415 (1269606) 1269606..1270226 - 621 WP_001267012.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T169435 WP_001291435.1 NZ_CP059929:1265181-1265399 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T169435 NZ_CP076484:c198249-197977 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT169435 WP_000344800.1 NZ_CP059929:1264781-1265155 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT169435 NZ_CP076484:c198512-198249 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References