Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 187014..187657 Replicon plasmid p1
Accession NZ_CP059918
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag H5C78_RS25840 Protein ID WP_001044768.1
Coordinates 187241..187657 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag H5C78_RS25835 Protein ID WP_001261287.1
Coordinates 187014..187244 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS25810 183321..184103 - 783 WP_001513526.1 site-specific integrase -
H5C78_RS25815 184104..184409 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H5C78_RS25820 184411..184629 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H5C78_RS25825 185185..185874 + 690 WP_001513525.1 helix-turn-helix domain-containing protein -
H5C78_RS25830 185906..186595 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
H5C78_RS25835 187014..187244 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H5C78_RS25840 187241..187657 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
H5C78_RS25845 187819..189957 - 2139 WP_001513523.1 AAA family ATPase -
H5C78_RS25850 190344..190574 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H5C78_RS25855 190571..190987 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H5C78_RS25860 191062..192291 + 1230 Protein_200 AAA family ATPase -
H5C78_RS25865 192369..192641 + 273 WP_000019163.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..214538 214538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T169429 WP_001044768.1 NZ_CP059918:187241-187657 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T169429 NZ_CP076477:545514-545855 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT169429 WP_001261287.1 NZ_CP059918:187014-187244 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT169429 NZ_CP076477:545284-545514 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References