Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 184104..184629 Replicon plasmid p1
Accession NZ_CP059918
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H5C78_RS25815 Protein ID WP_001159871.1
Coordinates 184104..184409 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H5C78_RS25820 Protein ID WP_000813630.1
Coordinates 184411..184629 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS25790 179283..179729 - 447 WP_053270998.1 hypothetical protein -
H5C78_RS25795 179894..181122 + 1229 WP_182917278.1 IS3-like element IS2 family transposase -
H5C78_RS25800 181125..181298 - 174 Protein_188 RepB family plasmid replication initiator protein -
H5C78_RS25805 181615..182823 - 1209 WP_000343717.1 IS256 family transposase -
H5C78_RS25810 183321..184103 - 783 WP_001513526.1 site-specific integrase -
H5C78_RS25815 184104..184409 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H5C78_RS25820 184411..184629 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H5C78_RS25825 185185..185874 + 690 WP_001513525.1 helix-turn-helix domain-containing protein -
H5C78_RS25830 185906..186595 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
H5C78_RS25835 187014..187244 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H5C78_RS25840 187241..187657 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..214538 214538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T169428 WP_001159871.1 NZ_CP059918:c184409-184104 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T169428 NZ_CP076477:202588-202854 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGACAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAGAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT169428 WP_000813630.1 NZ_CP059918:c184629-184411 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT169428 NZ_CP076477:202325-202588 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References