Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 122139..122764 Replicon plasmid p1
Accession NZ_CP059918
Organism Escherichia coli strain 64.1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag H5C78_RS25450 Protein ID WP_000911313.1
Coordinates 122366..122764 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID V0VCB2
Locus tag H5C78_RS25445 Protein ID WP_000450520.1
Coordinates 122139..122366 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5C78_RS25445 122139..122366 + 228 WP_000450520.1 toxin-antitoxin system antitoxin VapB Antitoxin
H5C78_RS25450 122366..122764 + 399 WP_000911313.1 tRNA(fMet)-specific endonuclease VapC Toxin
H5C78_RS25455 122773..124962 - 2190 WP_001611030.1 type IV conjugative transfer system coupling protein TraD -
H5C78_RS25460 125082..125507 + 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
H5C78_RS25465 125504..125854 + 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
H5C78_RS25470 125885..127498 + 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..214538 214538


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(8-50)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14901.15 Da        Isoelectric Point: 7.8605

>T169423 WP_000911313.1 NZ_CP059918:122366-122764 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Download         Length: 399 bp

>T169423 NZ_CP076470:4524877-4524984 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8596.63 Da        Isoelectric Point: 4.7513

>AT169423 WP_000450520.1 NZ_CP059918:122139-122366 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT169423 NZ_CP076470:c4524827-4524764 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB V0VCB2

References