Detailed information of TA system TA169418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 104294..104829
• Replicon: plasmid p1
• Accession: NZ_CP059918
• Organism: Escherichia coli strain 64.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: H5C78_RS25360
• Protein ID: WP_000222760.1
• Coordinates: 104294..104581 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: H5C78_RS25365
• Protein ID: WP_001132900.1
• Coordinates: 104578..104829 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5C78_RS25335	99877..101098	-	1222	Protein_95	ISL3 family transposase	-
H5C78_RS25340	101198..101562	+	365	Protein_96	IS1 family transposase	-
H5C78_RS25345	101617..102396	-	780	WP_001513481.1	IS21-like element IS100kyp family helper ATPase IstB	-
H5C78_RS25350	102396..103418	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
H5C78_RS25355	103507..103854	+	348	Protein_99	IS1-like element IS1A family transposase	-
H5C78_RS25360	104294..104581	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5C78_RS25365	104578..104829	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5C78_RS25370	105796..106653	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H5C78_RS25375	107157..108698	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H5C78_RS25380	108713..109459	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
H5C78_RS25385	109707..109781	-	75	WP_001442103.1	RepA leader peptide Tap	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..214538	214538
	-	inside	IScluster/Tn	-	-	100139..113585	13446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T169418 WP_000222760.1 NZ_CP059918:c104581-104294 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T169418 NZ_CP076470:4099374-4099661 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT169418 WP_001132900.1 NZ_CP059918:c104829-104578 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT169418 NZ_CP076470:4099135-4099374 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2